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|Antigen||Lymphoid Enhancer-Binding Factor 1 (LEF1) Antibodies|
|Epitope||HMG Binding Domain Alternatives|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This LEF1 antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Western Blotting (WB)
|1 reference available|
|Supplier||Log in to see|
Product Details anti-LEF1 AntibodyTarget Details LEF1 Application Details Handling References for anti-LEF1 antibody (ABIN264381) Images
|Specificity||REMB6 recognizes the High Mobility Group DNA domain of LEF-1.|
|Characteristics||Synonyms: Lymphoid enhancer-binding factor 1, LEF-1, T cell-specific transcription factor 1-alpha,TCF1-alpha|
|Immunogen||Full-length recombinant LEF-1 protein. Genename: LEF1|
Target Details LEF1Product Details anti-LEF1 Antibody Application Details Handling References for anti-LEF1 antibody (ABIN264381) Images back to top
|Alternative Name||LEF1 (LEF1 Antibody Abstract)|
|Background||Lymphoid Enhancer Factor-1 is a transcription factor of the High Mobility Group of DNA binding proteins. It is one member of a family of four proteins referred to as LEF/TCF transcription factors (LEF-1, TCF-1, TCF-3 and TCF-4). These factors play a crucial role in WNT/WIngless signaling, a signal transduction cascade that directs cell differentiation. Aberrant activation of the WNT/Wingless pathway is also a root cause in the genesis of certain cancers such as colon cancer, melanoma and breast cancer.LEF-1 is expressed during development in many different differentiating tissues, and in a few tissues after birth. LEF-1 expression is required for proper development of breast, teeth, hair, whiskers and the trigeminal nerve. It is redundant with TCF-1 (for T Cell Factor-1) for correct development of T lymphocytes in the thymus. LEF-1 is a 399 amino acid protein that appears as a cluster of polypeptides ranging in size between 55-57 kD on SDS-PAGE analysis. Alternative splice forms of LEF-1 and other LEF/TCF family members have been identified, but the expression pattern and relative abundance of these alternative forms have not been well studied. They can appear on westerns as smaller immunoreactive forms.Synonyms: LEF-1, Lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha, TCF1-alpha|
|Research Area||Transcription Factors, Wnt, Lineage Markers, Wnt Signaling|
|Pathways||WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding|
Application DetailsProduct Details anti-LEF1 Antibody Target Details LEF1 Handling References for anti-LEF1 antibody (ABIN264381) Images back to top
Western Blot: 1-5 μg/mLImmunofluorescence on Paraformaldehyde-Fixed Cells: 1 μg/mL (1/1000). Positive Control: Jurkat cell lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-LEF1 Antibody Target Details LEF1 Application Details References for anti-LEF1 antibody (ABIN264381) Images back to top
|Buffer||PBS, 0.08 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
|Expiry Date||12 months|
References for anti-LEF1 antibody (ABIN264381)Product Details anti-LEF1 Antibody Target Details LEF1 Application Details Handling Images back to top
Hsu, Galceran, Grosschedl: "Modulation of transcriptional regulation by LEF-1 in response to Wnt-1 signaling and association with beta-catenin." in: Molecular and cellular biology, Vol. 18, Issue 8, pp. 4807-18, 1998
ImagesProduct Details anti-LEF1 Antibody Target Details LEF1 Application Details Handling References for anti-LEF1 antibody (ABIN264381) back to top