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anti-Mouse (Murine) LEF1 antibody for Western Blotting

Recommended LEF1 Antibody (supplied by: Log in to see )

Antigen
Lymphoid Enhancer-Binding Factor 1 (LEF1) Antibodies
  • 3000002B05
  • AI451430
  • LEF-1
  • Lef-1
  • LEF1
  • TCF1ALPHA
  • TCF7L3
  • TCF10
Epitope
N-Term
20
16
16
6
6
6
6
6
4
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
122
33
25
7
3
3
2
2
1
1
1
1
1
Host
Rabbit
53
48
17
5
Clonality
Polyclonal
Conjugate
This LEF1 antibody is un-conjugated
3
3
3
3
3
3
Application
Western Blotting (WB)
112
65
21
14
6
5
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN2792635
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12917753 ABIN2779620 IHC WB Rabbit C-Term Log in to see Polyclonal 1
0.12917753 ABIN264381 IF WB Mouse IgG1 HMG Binding Domain Log in to see REMB6 1
0.12917753 ABIN188546 ELISA WB Goat IgG Log in to see Polyclonal 2
0.12917753 ABIN2779621 WB Rabbit Middle Region Log in to see Polyclonal
0.12917753 ABIN735248 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal 3
0.12917753 ABIN1531685 ELISA WB Rabbit IgG pSer42 Log in to see Polyclonal
0.12917753 ABIN2465242 ELISA WB Goat Internal Region Log in to see Polyclonal
0.12917753 ABIN2460458 IHC ELISA WB Rabbit Log in to see Polyclonal
0.12917753 ABIN2491171 ELISA WB Rabbit IgG pSer42 Log in to see Polyclonal
0.12917753 ABIN264379 IF IHC (p) WB Mouse IgG1 Log in to see REMB1
0.12917753 ABIN3182271 ELISA WB Rabbit IgG pSer42 Log in to see Polyclonal
0.12917753 ABIN2487470 WB Goat Log in to see Polyclonal
0.0011819942 ABIN782977 WB Goat Internal Region Log in to see Polyclonal 1
0.0011819942 ABIN185607 ELISA WB Goat Internal Region Log in to see Polyclonal 2
0.0011819942 ABIN374666 WB Goat Internal Region Log in to see Polyclonal 1
0.0011819942 ABIN1387756 IF (p) IHC (p) WB Rabbit IgG pSer42 Log in to see Polyclonal 1
0.0011819942 ABIN793575 ICC IHC WB Mouse IgG1 Log in to see REMB6
0.0011819942 ABIN1584697 IHC ELISA WB Rabbit Log in to see Polyclonal
0.0011819942 ABIN1088251 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011819942 ABIN1088252 IHC ELISA WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-LEF1 antibody for Western Blotting

Similar anti-LEF1 Antibodies

Application / Reactivity Human Cow (Bovine) Mouse (Murine) Pig (Porcine) Rabbit Rat (Rattus)
Western Blotting (WB) 112 Antibodies 4 Antibodies 29 Antibodies 4 Antibodies 3 Antibodies 21 Antibodies
RNA Interference (RNAi) 1 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 6 Antibodies 3 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 14 Antibodies 1 Antibodies 8 Antibodies 1 Antibodies 1 Antibodies 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 2 Antibodies 2 Antibodies 1 Antibodies

General

Antigen Lymphoid Enhancer-Binding Factor 1 (LEF1) Antibodies
Epitope N-Term
(20), (16), (16), (6), (6), (6), (6), (6), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(122), (33), (25), (7), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(53), (48), (17), (5)
Clonality
Polyclonal
Conjugate This LEF1 antibody is un-conjugated
(3), (3), (3), (3), (3), (3)
Application Western Blotting (WB)
(112), (65), (21), (14), (6), (5), (3), (2), (2), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-LEF1 Antibody

Target Details LEF1 Application Details Handling References for anti-LEF1 Antibody (ABIN2792635) Images
Sequence VARQAQTSQE PYHDKAREHP DDGKHPDGGL YNKGPSYSSY SGYIMMPNMN
Cross-Reactivity Cow (Bovine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
Predicted Reactivity Horse : 100 %, Human : 100 %, Bovine : 90 %, Dog : 90 %, Rat : 90 %, Guinea pig : 80 %, Mouse : 80 %, Pig : 80 %
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human LEF1

Target Details LEF1

Product Details anti-LEF1 Antibody Application Details Handling References for anti-LEF1 Antibody (ABIN2792635) Images back to top
Antigen
Alternative Name LEF1 (LEF1 Antibody Abstract)
Background Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1). Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA, MIM 186880) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1, MIM 163905).
Molecular Weight 44 kDa
Gene ID 51176
NCBI Accession NP_057353, NM_016269
UniProt Q9UJU2
Research Area Transcription Factors, Wnt, Lineage Markers
Pathways WNT Signaling

Application Details

Product Details anti-LEF1 Antibody Target Details LEF1 Handling References for anti-LEF1 Antibody (ABIN2792635) Images back to top
Application Notes WB Suggested Anti-LEF1 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:312500
Positive Control: Hela cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 399 AA

Restrictions For Research Use only

Handling

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details References for anti-LEF1 Antibody (ABIN2792635) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-LEF1 Antibody (ABIN2792635)

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details Handling Images back to top
Background publications

Petropoulos, Arseni, Schessl, Stadler, Rawat, Deshpande, Heilmeier, Hiddemann, Quintanilla-Martinez, Bohlander, Feuring-Buske, Buske: "A novel role for Lef-1, a central transcription mediator of Wnt signaling, in leukemogenesis." in: The Journal of experimental medicine, Vol. 205, Issue 3, pp. 515-22, 2008

Images

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details Handling References for anti-LEF1 Antibody (ABIN2792635) back to top
Supplier Images
Western Blotting (WB) image for anti-LEF1 antibody (Lymphoid Enhancer-Binding Factor 1) (N-Term) (ABIN2792635) WB Suggested Anti-LEF1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...