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|Antigen||phospholipase C, beta 1 (phosphoinositide-Specific) (PLCB1) Antibodies|
|Conjugate||This Phospholipase C beta 1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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Product Details anti-Phospholipase C beta 1 AntibodyTarget Details Phospholipase C beta 1 Application Details Handling Images
|Characteristics||Cellular Localization: Cytoplasm, Membrane|
|Purification||Immunogen Affinity Purified|
|Immunogen||Synthetic peptide corresponding to C-terminus of human PLC beta 1.|
Target Details Phospholipase C beta 1Product Details anti-Phospholipase C beta 1 Antibody Application Details Handling Images back to top
|Alternative Name||PLC beta 1 (PLCB1 Antibody Abstract)|
|Background||Phosphoinositide-specific phospholipase C (PLC) plays a crucial role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5- triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. There are many mammalian PLC isozymes, including PLC β1, PLC β2, PLC β3, PLC β4, PLC γ1, PLC γ2, PLC δ1, PLC δ2 and PLC ε, with molecular weights ranging from 85 to 150kDa. PLC β1, one of the PLC β isozymes, exists as two immunologically distinguishable proteins of 150 (PLC-β1a) and 140kDa (PLC β1b). The two isoforms encode in two distinct transcripts and generated by alternative splicing of a single gene. PLC β1a is preferentially expressed in the cytosol, whereas PLC β1b is predominantly localized in the nuclei. PLC β1 is a G-protein-dependent phosphodiesterase that hydrolyses phosphatidylinositol 4,5 biphosphate into inositol 1,4,5-triphosphate and diacylglycerol after the stimulation of a variety of neurotransmitter receptors at the cell surface.|
|Molecular Weight||150 kDa|
|Pathways||WNT Signaling, AMPK Signaling, Thyroid Hormone Synthesis, Inositol Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Proton Transport, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, G-protein mediated Events|
Application DetailsProduct Details anti-Phospholipase C beta 1 Antibody Target Details Phospholipase C beta 1 Handling Images back to top
|Application Notes||IHC Dilution: Ready to use, for IHC only|
IHC Procedure: Specimen
Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody.
Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols.
Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:25. The dilutions are estimates, actual results may differ because of variability in methods and protocols.
Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.
Primary Antibody Incubation: Incubate for 30 minutes at room temperature.
Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05 % Tween.
Visualization: Detect the antibody as instructed by the instructions provided with the visualization system.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Phospholipase C beta 1 Antibody Target Details Phospholipase C beta 1 Application Details Images back to top
|Buffer||pre-diluted immunogen affinity purified rabbit polyclonal antibody in TBS/1 % BSA buffer pH 7.6 with less than 0.1 % sodium azide.|
|Precaution of Use||
1. Avoid contact of reagents with eyes and mucous membranes. If reagents come into contact with sensitive areas, wash with copious amounts of water.
2. This product is harmful if swallowed.
3. Consult local or state authorities with regard to recommended method of disposal.
4. Avoid microbial contamination of reagents.
This product contains Sodium AzideTM: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
|Handling Advice||Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product, therefore, positive and negative controls should be tested simultaneously with unknown specimens.|
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