anti-Human Phospholipase C beta 1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Phospholipase C beta 1 Antibody (supplied by: Log in to see )

Antigen
phospholipase C, beta 1 (phosphoinositide-Specific) (PLCB1) Antibodies
  • PLCB1
  • 3110043I21Rik
  • AI132408
  • Plcb
  • mKIAA0581
  • EIEE12
  • PI-PLC
  • PLC-154
  • PLC-I
  • PLC154
  • PLCB1A
  • PLCB1B
  • PLC'1
  • PLCbeta1
  • Phosphb
  • RATPHOSPHB
  • phospholipase C, beta 1 (phosphoinositide-specific)
  • phospholipase C, beta 1
  • PLCB1
  • plcb1
  • Plcb1
Epitope
C-Term
15
4
3
2
1
1
1
1
1
1
Reactivity
Human
54
40
38
6
4
4
4
3
2
2
1
1
Host
Rabbit
51
4
1
Clonality
Polyclonal
Conjugate
This Phospholipase C beta 1 antibody is un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
34
16
13
10
7
5
3
2
1
1
1
Supplier
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Catalog No. ABIN1688638
$ 231.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1385859 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN1688634 IHC (p) Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN1415619 IHC (p) WB HRP Rabbit IgG Log in to see Polyclonal
1 ABIN1397746 IHC (p) WB Biotin Rabbit IgG Log in to see Polyclonal
1 ABIN5552547 IHC (p) Rabbit C-Term Log in to see Polyclonal
1 ABIN4346112 IHC IHC (p) Rabbit Log in to see Polyclonal
1 ABIN4346113 IHC IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN4346110 IHC IHC (p) Rabbit C-Term Log in to see Polyclonal
1 ABIN4346109 IHC IHC (p) Rabbit C-Term Log in to see Polyclonal
1 ABIN4346111 IHC IHC (p) Rabbit C-Term Log in to see Polyclonal

General

Antigen phospholipase C, beta 1 (phosphoinositide-Specific) (PLCB1) Antibodies
Epitope C-Term
(15), (4), (3), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(54), (40), (38), (6), (4), (4), (4), (3), (2), (2), (1), (1)
Host Rabbit
(51), (4), (1)
Clonality
Polyclonal
Conjugate This Phospholipase C beta 1 antibody is un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(34), (16), (13), (10), (7), (5), (3), (2), (1), (1), (1)
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Product Details anti-Phospholipase C beta 1 Antibody

Target Details Phospholipase C beta 1 Application Details Handling Images
Characteristics Cellular Localization: Cytoplasm, Membrane
Purification Immunogen Affinity Purified
Immunogen Synthetic peptide corresponding to C-terminus of human PLC beta 1.
Isotype IgG

Target Details Phospholipase C beta 1

Product Details anti-Phospholipase C beta 1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name PLC beta 1 (PLCB1 Antibody Abstract)
Background Phosphoinositide-specific phospholipase C (PLC) plays a crucial role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5- triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. There are many mammalian PLC isozymes, including PLC β1, PLC β2, PLC β3, PLC β4, PLC γ1, PLC γ2, PLC δ1, PLC δ2 and PLC ε, with molecular weights ranging from 85 to 150kDa. PLC β1, one of the PLC β isozymes, exists as two immunologically distinguishable proteins of 150 (PLC-β1a) and 140kDa (PLC β1b). The two isoforms encode in two distinct transcripts and generated by alternative splicing of a single gene. PLC β1a is preferentially expressed in the cytosol, whereas PLC β1b is predominantly localized in the nuclei. PLC β1 is a G-protein-dependent phosphodiesterase that hydrolyses phosphatidylinositol 4,5 biphosphate into inositol 1,4,5-triphosphate and diacylglycerol after the stimulation of a variety of neurotransmitter receptors at the cell surface.
Molecular Weight 150 kDa
Gene ID 23236
Pathways WNT Signaling, AMPK Signaling, Thyroid Hormone Synthesis, Inositol Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Proton Transport, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, G-protein mediated Events

Application Details

Product Details anti-Phospholipase C beta 1 Antibody Target Details Phospholipase C beta 1 Handling Images back to top
Application Notes IHC Dilution: Ready to use, for IHC only
Comment

Positive Control:
IHC (p): Brain

Protocol IHC Procedure: Specimen
Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody.
Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols.
Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:25. The dilutions are estimates, actual results may differ because of variability in methods and protocols.
Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.
Primary Antibody Incubation: Incubate for 30 minutes at room temperature.
Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05 % Tween.
Visualization: Detect the antibody as instructed by the instructions provided with the visualization system.
Restrictions For Research Use only

Handling

Product Details anti-Phospholipase C beta 1 Antibody Target Details Phospholipase C beta 1 Application Details Images back to top
Format Liquid
Buffer pre-diluted immunogen affinity purified rabbit polyclonal antibody in TBS/1 % BSA buffer pH 7.6 with less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use 1. Avoid contact of reagents with eyes and mucous membranes. If reagents come into contact with sensitive areas, wash with copious amounts of water.
2. This product is harmful if swallowed.
3. Consult local or state authorities with regard to recommended method of disposal.
4. Avoid microbial contamination of reagents.
This product contains Sodium AzideTM: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product, therefore, positive and negative controls should be tested simultaneously with unknown specimens.
Storage 4 °C

Images

Product Details anti-Phospholipase C beta 1 Antibody Target Details Phospholipase C beta 1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-phospholipase C, beta 1 (phosphoinositide-Specific) (PLCB1) (C-Term) antibody (ABIN1688638) anti-phospholipase C, beta 1 (phosphoinositide-Specific) (PLCB1) (C-Term) antibody