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anti-Human PKC alpha antibody for Immunofluorescence

Recommended PKC alpha Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, alpha (PKCa) Antibodies
  • AAG6
  • AI875142
  • im:7139045
  • LOC100219195
  • pkc-alpha
  • PKC-alpha
  • Pkca
  • PKCA
  • pkcalpha
  • PKRCA
  • PRKACA
  • si:dkey-8l19.1
Epitope
AA 270-427
49
34
17
15
15
13
11
10
7
5
5
3
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus)
228
114
91
8
4
3
2
2
2
1
1
1
1
1
Host
Mouse
214
26
2
Clonality
Monoclonal
Conjugate
This PKC alpha antibody is un-conjugated
10
9
7
6
5
5
4
4
2
2
2
2
2
2
2
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
185
107
89
35
33
22
21
19
6
5
4
4
2
1
1
1
1
1
1
1
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Catalog No. ABIN967750
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.32042634 ABIN967751 IF IP IHC WB Mouse IgG2b, kappa AA 270-427 Log in to see 3-PKCalpha 5
0.32042634 ABIN189676 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG C-Term Log in to see Y124
0.32042634 ABIN189675 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG N-Term Log in to see Y143
0.32042634 ABIN2495478 FACS IF Rabbit Ig Fraction C-Term Log in to see Polyclonal
0.32042634 ABIN562379 IF IHC (p) PLA ELISA WB Mouse IgG1 kappa AA 563-672, partial Log in to see 2F11
0.0010838947 ABIN391001 FACS IF IHC (p) WB Rabbit Ig AA 299-329, C-Term Log in to see Polyclonal 1
0.0010838947 ABIN272065 EIA IF IHC (p) WB Rabbit Log in to see Polyclonal
0.0010838947 ABIN189677 ICC IF IHC (p) IP IHC WB Rabbit IgG Thr638 Log in to see E195
0.0010838947 ABIN652517 FACS IF IHC (p) WB Rabbit Ig AA 75-103, N-Term Log in to see Polyclonal
0.0010838947 ABIN393606 IF WB Mouse IgG Mix kappa AA 563-673 Log in to see 2F11
0.0010838947 ABIN598889 IF ELISA WB Mouse IgG1, kappa Log in to see 2F11
0.0010838947 ABIN2887792 IF WB Rabbit IgG C-Term Log in to see Polyclonal
0.0010838947 ABIN220833 ICC FACS IF IP WB Rabbit Log in to see Polyclonal
0.0010838947 ABIN408073 ICC IF IHC (p) IHC WB Rabbit IgG Thr495 Log in to see Polyclonal
0.0010838947 ABIN1926582 FACS IF IHC (p) WB Rabbit N-Term, AA 75-103 Log in to see Polyclonal
0.0010838947 ABIN1841091 FACS IF IHC (p) WB Rabbit C-Term Log in to see Polyclonal
0.0010838947 ABIN2499394 FACS IF IP IHC WB Mouse IgG2a Log in to see MC5
0.0010838947 ABIN3032373 FACS IF IHC ELISA WB Rabbit Ig Fraction AA 299-329 Log in to see Polyclonal
0.0010838947 ABIN222041 IF ELISA WB Rabbit Log in to see Polyclonal
0.0010838947 ABIN2336405 FACS IF IHC WB Rabbit AA 75-103 Log in to see Polyclonal

Top referenced anti-PKC alpha antibody for Immunofluorescence

Similar anti-PKC alpha Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Chicken Dog (Canine) Frog
Western Blotting (WB) 181 Antibodies 80 Antibodies 68 Antibodies 2 Antibodies 3 Antibodies 2 Antibodies
Radioimmunoassay (RIA) 1 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Intracellular Staining (ICS) 5 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies 4 Antibodies 1 Antibodies
Immunoprecipitation (IP) 24 Antibodies 10 Antibodies

General

Antigen Protein Kinase C, alpha (PKCa) Antibodies
Epitope AA 270-427
(49), (34), (17), (15), (15), (13), (11), (10), (7), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus)
(228), (114), (91), (8), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(214), (26), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PKC alpha antibody is un-conjugated
(10), (9), (7), (6), (5), (5), (4), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(185), (107), (89), (35), (33), (22), (21), (19), (6), (5), (4), (4), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-PKC alpha Antibody

Target Details PKC alpha Application Details Handling References for anti-PKC alpha Antibody (ABIN967750) Images
Cross-Reactivity Rat (Rattus), Mouse (Murine), Chicken, Dog (Canine), Frog
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human PKCalpha aa. 270-427
Clone 3-PKCalpha
Isotype IgG2b, kappa

Target Details PKC alpha

Product Details anti-PKC alpha Antibody Application Details Handling References for anti-PKC alpha Antibody (ABIN967750) Images back to top
Antigen
Alternative Name PKC alpha (PKCa Antibody Abstract)
Background The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. These proteins are products of multiple genes and alternative splicing. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half containing C1, C2, V1, and V2 constitutes the regulatory domain and interacts with PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester. However, the novel PKC (nPKC) subfamily members ( delta, epsilon, eta, and theta isoforms) and the atypical PKC (aPKC) subfamily members (zeta, iota, and lambda isoforms) are Ca2+ independent and lack the C2 domain. The aPKC members are unique in that their activity is independent of diacylglycerols and phorbol esters. They also lack one repeat of the cysteine-rich sequences that are conserved in cPKC and nPKC. The C-terminal region of PKC contains the catalytic domain. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters and growth factors. Overexpression of PKCalpha has been reported to lead to an enhanced growth rate and induces the phosphorylation of two cellular proteins of 52 kDa and 90 kDa. This antibody is routinely tested by western blot analysis.
This antibody has been reported to also crossreact with PKCbeta.
Synonyms: Protein Kinase Calpha
Molecular Weight 82 kDa
Research Area Cardiovascular, Hypertrophy, Signaling, Protein Modifications, Kinases/Phosphatases
Pathways WNT Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-PKC alpha Antibody Target Details PKC alpha Handling References for anti-PKC alpha Antibody (ABIN967750) Images back to top
Comment

Related Products: ABIN968545, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details References for anti-PKC alpha Antibody (ABIN967750) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

References for anti-PKC alpha Antibody (ABIN967750)

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Handling Images back to top
Product cited in:

Fujino, Wu, Lin, Phillips, Nedivi: "cpg15 and cpg15-2 constitute a family of activity-regulated ligands expressed differentially in the nervous system to promote neurite growth and neuronal survival." in: The Journal of comparative neurology, Vol. 507, Issue 5, pp. 1831-45, 2008

Powner, Hodgkin, Wakelam: "Antigen-stimulated activation of phospholipase D1b by Rac1, ARF6, and PKCalpha in RBL-2H3 cells." in: Molecular biology of the cell, Vol. 13, Issue 4, pp. 1252-62, 2002

Wagner, Fowler, Takemoto: "The interaction and phosphorylation of tropomodulin by protein kinase Calpha in N/N 1003A lens epithelial cells." in: Molecular vision, Vol. 8, pp. 394-406, 2002

Zhang, Bontrager, Hemler: "Transmembrane-4 superfamily proteins associate with activated protein kinase C (PKC) and link PKC to specific beta(1) integrins." in: The Journal of biological chemistry, Vol. 276, Issue 27, pp. 25005-13, 2001

Smart, Ying, Anderson: "Hormonal regulation of caveolae internalization." in: The Journal of cell biology, Vol. 131, Issue 4, pp. 929-38, 1996

Nishizuka: "The molecular heterogeneity of protein kinase C and its implications for cellular regulation." in: Nature, Vol. 334, Issue 6184, pp. 661-5, 1988

Images

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Handling References for anti-PKC alpha Antibody (ABIN967750) back to top
Supplier Images
Western Blotting (WB) image for anti-PKC alpha antibody (Protein Kinase C, alpha) (AA 270-427) (ABIN967750) Western blot analysis of PKCalpha on a rat cerebrum lysate. Lane 1: 1:1000, lane 2: 1...
Immunofluorescence (IF) image for anti-PKC alpha antibody (Protein Kinase C, alpha) (AA 270-427) (ABIN967750) Immunofluorescence staining of PC12 cells (Rat neuroblastoma, ATCC CRL-1721).