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anti-Human PKC alpha antibody for Western Blotting

Recommended PKC alpha Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, alpha (PKCa) Antibodies
  • pkc-alpha
  • pkcalpha
  • im:7139045
  • si:dkey-8l19.1
  • LOC100219195
  • AAG6
  • PKC-alpha
  • PKCA
  • PRKACA
  • AI875142
  • Pkca
  • PKRCA
  • protein kinase C, alpha
  • PRKCA
  • prkca
  • LOC100219195
  • Prkca
Epitope
AA 299-329, C-Term
43
26
17
16
16
15
12
10
7
5
5
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
257
185
147
29
25
23
17
17
16
5
4
3
3
3
2
1
1
1
Host
Rabbit
222
43
2
Clonality
Polyclonal
Conjugate
This PKC alpha antibody is un-conjugated
11
10
8
7
5
5
5
5
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
211
117
94
58
45
36
27
26
21
19
5
4
4
3
2
1
1
1
1
Options
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Catalog No. ABIN391001
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0923712 ABIN1531462 IHC ELISA WB Rabbit IgG pThr638 Log in to see Polyclonal 1
3.0923712 ABIN359058 EIA IHC (p) WB Rabbit Ig C-Term Log in to see Polyclonal 5
3.0923712 ABIN453789 EIA WB Rabbit N-Term Log in to see Polyclonal 2
3.0923712 ABIN967751 IF IP IHC WB Mouse IgG2b, kappa AA 270-427 Log in to see 3-PKCalpha 5
3.0923712 ABIN967750 IF IP IHC WB Mouse IgG2b, kappa AA 270-427 Log in to see 3-PKCalpha 6
3.0923712 ABIN3043549 WB Rabbit IgG AA 153-342 Log in to see Polyclonal
3.0923712 ABIN658198 FACS IHC (p) WB Rabbit Ig AA 1-30, N-Term Log in to see Polyclonal
3.0923712 ABIN1531505 ELISA WB Rabbit IgG pTyr657 Log in to see Polyclonal
3.0923712 ABIN3043025 IHC (p) WB Rabbit IgG AA 153-182, N-Term Log in to see Polyclonal
3.0923712 ABIN1108731 IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
3.0923712 ABIN3186466 ELISA IHC WB Rabbit IgG Thr287 Log in to see Polyclonal
3.0923712 ABIN2856799 IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
3.0923712 ABIN652517 FACS IF IHC (p) WB Rabbit Ig AA 75-103, N-Term Log in to see Polyclonal
3.0923712 ABIN3182587 ELISA IHC WB Rabbit IgG pThr638 Log in to see Polyclonal
3.0923712 ABIN3182236 ELISA WB Rabbit IgG pTyr657 Log in to see Polyclonal
3.0923712 ABIN1532520 ELISA WB Rabbit IgG Log in to see Polyclonal
3.0923712 ABIN1532477 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
3.0923712 ABIN498990 WB Rabbit Log in to see Polyclonal
3.0923712 ABIN256441 IP WB Mouse IgG2a kappa Log in to see PKC0103
3.0923712 ABIN393606 IF WB Mouse IgG Mix kappa AA 563-673 Log in to see 2F11

Top referenced anti-PKC alpha antibody for Western Blotting

Similar anti-PKC alpha Antibodies

Application / Reactivity Human
ELISA 111 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 45 Antibodies
Functional Studies (Func) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 20 Antibodies
Immunofluorescence (IF) 37 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 89 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 17 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 59 Antibodies
Immunoprecipitation (IP) 26 Antibodies
Intracellular Staining (ICS) 5 Antibodies
Neutralization (Neut) 1 Antibodies

General

Antigen Protein Kinase C, alpha (PKCa) Antibodies
Epitope AA 299-329, C-Term
(43), (26), (17), (16), (16), (15), (12), (10), (7), (5), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(257), (185), (147), (29), (25), (23), (17), (17), (16), (5), (4), (3), (3), (3), (2), (1), (1), (1)
Host Rabbit
(222), (43), (2)
Clonality (Clone)
Polyclonal   ( )
Conjugate This PKC alpha antibody is un-conjugated
(11), (10), (8), (7), (5), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(211), (117), (94), (58), (45), (36), (27), (26), (21), (19), (5), (4), (4), (3), (2), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-PKC alpha Antibody

Target Details PKC alpha Application Details Handling References for anti-PKC alpha Antibody (ABIN391001) Images
Specificity This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PKC.
Predicted Reactivity Mouse (Murine),Rat (Rattus),Rabbit
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This PKC alpha antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 299-329 AA from the C-terminal region of human PKC alpha.
Clone RB01012
Isotype Ig

Target Details PKC alpha

Product Details anti-PKC alpha Antibody Application Details Handling References for anti-PKC alpha Antibody (ABIN391001) Images back to top
Antigen
Alternative Name PKC alpha (PKCa Antibody Abstract)
Background Synonyms: Protein kinase C, alpha type, PRKCA, PKCA, PRKACA
Pathways: Fc gamma R-mediated phagocytosis, MAPK signaling pathway, VEGF signaling pathway, Fc epsilon RI signaling pathway, Long-term depression, GnRH signaling pathway, Pancreatic secretion, Pathways in cancer, Non-small cell lung cancer, Calcium signaling pathway, Phosphatidylinositol signaling system, Natural killer cell mediated cytotoxicity, Focal adhesion, Endocrine and other factor-regulated calcium reabsorption, Pathogenic Escherichia coli infection, Wnt signaling pathway, Aldosterone-regulated sodium reabsorption, Gap junction, Melanogenesis, Tight junction, ErbB signaling pathway, Amoebiasis, Glioma
Molecular Weight 76764 Da
Gene ID 5578
UniProt P17252
Research Area Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Cycle, Cell Structure
Pathways WNT Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling

Application Details

Product Details anti-PKC alpha Antibody Target Details PKC alpha Handling References for anti-PKC alpha Antibody (ABIN391001) Images back to top
Application Notes IF = 1:10-50, FACS = 1:10-50, WB = 1:1000, IHC (p) = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details References for anti-PKC alpha Antibody (ABIN391001) Images back to top
Format Liquid
Concentration 1.79 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-PKC alpha Antibody (ABIN391001)

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Handling Images back to top
Product cited in:

Jaafar, Zeiller, Pirola, Di Grazia, Naro, Vidal, Lefai, Némoz: "Phospholipase D regulates myogenic differentiation through the activation of both mTORC1 and mTORC2 complexes." in: The Journal of biological chemistry, Vol. 286, Issue 25, pp. 22609-21, 2011

Images

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Handling References for anti-PKC alpha Antibody (ABIN391001) back to top
Supplier Images
Western Blotting (WB) image for anti-PKC alpha antibody (Protein Kinase C, alpha) (AA 299-329) (ABIN391001) Western blot analysis of anti-PKCalpha Pab (ABIN391001) in placenta lysate. PKCalpha ...
Immunohistochemistry (IHC) image for anti-PKC alpha antibody (Protein Kinase C, alpha) (AA 299-329) (ABIN391001) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...