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|Antigen||Protein Kinase C, alpha (PKCa) Antibodies|
|Reactivity||Human, Mammalian Alternatives|
|Conjugate||This PKC alpha antibody is un-conjugated Alternatives|
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-PKC alpha AntibodyTarget Details PKC alpha Application Details Handling Images
|Specificity||PKC alpha antibody (Cat#SM1436) recognises the alpha isoform of Protein Kinase C. It recognises the sequence PQFVHPILQSAV at the C terminus of PKCa.|
|Immunogen||Protein Kinase C alpha (PKC alpha). Spleen cells from immunised mice were fused with cells of the mouse SP2/0 myeloma cell line.|
Target Details PKC alphaProduct Details anti-PKC alpha Antibody Application Details Handling Images back to top
|Alternative Name||PRKCA (PKCa Antibody Abstract)|
|Background||Protein Kinase c alpha (PKC alpha) is an 77 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol, phosphatidylserine and phorbol esters) of the PKC family of serine/ threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC alpha is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of a broad range of cellular functions including proliferation, differentiation, development, migration, cell cell adhesion, cell extracellular matrix adhesion, and solute transport. The activation loop threonine (threonine 497 in PKC alpha) of conventional PKCs is phosphorylated by phosphoinositide dependent kinase 1 (PDK1). This phosphorylation is necessary for the autophosphorylation of threonine 638 in the carboxy terminus of PKC alpha, a step that is critical for regulating the rate of PKC alpha dephosphorylation and inactivation.Synonyms: PKC alpha, PKC-A, PKC-alpha, PKCA, PRKACA, Protein kinase C alpha type|
|Research Area||Cardiovascular, Hypertrophy, Kinases/Phosphatases|
|Pathways||WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling|
Application DetailsProduct Details anti-PKC alpha Antibody Target Details PKC alpha Handling Images back to top
Immunohistochemistry on frozen sections: Neat - 1/10. Western Blot: 1/10 - 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Images back to top
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|