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anti-Mouse (Murine) PKC alpha antibody for Immunocytochemistry

Recommended PKC alpha Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, alpha (PKCa) Antibodies
  • AAG6
  • AI875142
  • im:7139045
  • LOC100219195
  • pkc-alpha
  • PKC-alpha
  • Pkca
  • PKCA
  • pkcalpha
  • PKRCA
  • PRKACA
  • si:dkey-8l19.1
Epitope
Thr638
49
34
17
15
15
13
11
10
7
5
5
4
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
229
115
92
8
4
4
3
3
2
1
1
1
1
1
Host
Rabbit
214
27
2
Clonality
Monoclonal
Conjugate
This PKC alpha antibody is un-conjugated
10
9
7
6
5
5
4
4
2
2
2
2
2
2
2
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
186
107
90
35
33
22
22
20
6
5
4
4
2
1
1
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN189677
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010155214 ABIN189675 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG N-Term Log in to see Y143
0.0010155214 ABIN1175207 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 339-597 Log in to see Polyclonal
0.0010155214 ABIN408073 ICC IF IHC (p) IHC WB Rabbit IgG Thr495 Log in to see Polyclonal

Similar anti-PKC alpha Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 31 Antibodies 36 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 2 Antibodies
Flow Cytometry (FACS) 2 Antibodies 11 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies 4 Antibodies
Immunofluorescence (IF) 7 Antibodies 9 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies 22 Antibodies
Immunohistochemistry (IHC) 36 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 15 Antibodies
Immunoprecipitation (IP) 10 Antibodies
Intracellular Staining (ICS) 1 Antibodies
Western Blotting (WB) 68 Antibodies

General

Antigen Protein Kinase C, alpha (PKCa) Antibodies
Epitope Thr638
(49), (34), (17), (15), (15), (13), (11), (10), (7), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(229), (115), (92), (8), (4), (4), (3), (3), (2), (1), (1), (1), (1), (1)
Host Rabbit
(214), (27), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PKC alpha antibody is un-conjugated
(10), (9), (7), (6), (5), (5), (4), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(186), (107), (90), (35), (33), (22), (22), (20), (6), (5), (4), (4), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-PKC alpha Antibody

Target Details PKC alpha Application Details Handling Images
Specificity The antibody only detects PKC alpha phosphorylated on Threonine 638.
Cross-Reactivity (Details) Predicted to work with: Chicken, Bovine.
Purification Protein A purified
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Thr638 of human PKCa was used as immunogen.
Clone E195
Isotype IgG

Target Details PKC alpha

Product Details anti-PKC alpha Antibody Application Details Handling Images back to top
Antigen
Alternative Name PRKCA (PKCa Antibody Abstract)
Background Activation of PKC plays a crucial role in tranducing signals for a variety of cellularresponses, including tumor proliferation (1). PKC isoforms have been classified intothree groups: classical, novel, and atypical. Members of these three PKC groups containan autoinhibitory domain that binds to the substrate binding site in the catalytic domain,preventing its activation in the absence of cofactors or activators (2,3). The activity ofPKC is under the control of three distinct phosphorylation events, including that of theThr641 autophosphorylation site (4). Alternate Names: anti-AAG6 antibody, anti-MGC129900 antibody, anti-MGC129901 antibody, anti-PICK 1antibody, anti-PKC A antibody, anti-PKC alpha antibody, anti-PKCA antibody,anti-PRKACA antibody, anti-PRKC A antibody, anti-PRKCA antibody, anti-ProteinKinase C alpha antibody, anti-Protein kinase C alpha type antibody.
Gene Symbol: PRKCA
Gene ID 5578, 18750, 25636
Pathways WNT Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-PKC alpha Antibody Target Details PKC alpha Handling Images back to top
Application Notes This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation. In Western blot this antibody detects a band at approximately 80kDa.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:500, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:250, Immunoprecipitation 1:40, Western Blot 1:20000
Protocol Protocol specific for PKC alpha Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-PKC alpha antibody (Protein Kinase C, alpha) (Thr638) (ABIN189677) Western blot analysis on 293 cell lysate using anti-Phospho-PKCa (pT638) RabMAb (cat....
Immunohistochemistry (IHC) image for anti-PKC alpha antibody (Protein Kinase C, alpha) (Thr638) (ABIN189677) Immunohistochemical staining of (A) untreated and (B) Phosphatase-treated paraffin-em...
Immunohistochemistry (IHC) image for anti-PKC alpha antibody (Protein Kinase C, alpha) (Thr638) (ABIN189677) anti-Protein Kinase C, alpha (PKCa) (Thr638) antibody (Image 3)