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anti-Rat (Rattus) PKC alpha antibody for Flow Cytometry

Recommended PKC alpha Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, alpha (PKCa) Antibodies
  • AAG6
  • AI875142
  • im:7139045
  • LOC100219195
  • pkc-alpha
  • PKC-alpha
  • Pkca
  • PKCA
  • pkcalpha
  • PKRCA
  • PRKACA
  • si:dkey-8l19.1
Epitope
N-Term
49
34
17
15
15
13
11
10
7
5
5
4
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
229
115
92
8
4
4
3
3
2
1
1
1
1
1
Host
Rabbit
214
27
2
Clonality
Monoclonal
Conjugate
This PKC alpha antibody is un-conjugated
10
9
7
6
5
5
4
4
2
2
2
2
2
2
2
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
186
107
90
35
33
22
22
20
6
5
4
4
2
1
1
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN189675
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010660065 ABIN2499394 FACS IF IP IHC WB Mouse IgG2a Log in to see MC5

Similar anti-PKC alpha Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 31 Antibodies 36 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 2 Antibodies
Flow Cytometry (FACS) 2 Antibodies 11 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies 4 Antibodies
Immunofluorescence (IF) 7 Antibodies 9 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies 22 Antibodies
Immunohistochemistry (IHC) 36 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 15 Antibodies
Immunoprecipitation (IP) 10 Antibodies
Intracellular Staining (ICS) 1 Antibodies
Western Blotting (WB) 68 Antibodies

General

Antigen Protein Kinase C, alpha (PKCa) Antibodies
Epitope N-Term
(49), (34), (17), (15), (15), (13), (11), (10), (7), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(229), (115), (92), (8), (4), (4), (3), (3), (2), (1), (1), (1), (1), (1)
Host Rabbit
(214), (27), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PKC alpha antibody is un-conjugated
(10), (9), (7), (6), (5), (5), (4), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(186), (107), (90), (35), (33), (22), (22), (20), (6), (5), (4), (4), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-PKC alpha Antibody

Target Details PKC alpha Application Details Handling Images
Purification IgG purified
Immunogen A synthetic peptide corresponding to residues near the N-terminus of human PKC a was used as immunogen.
Clone Y143
Isotype IgG

Target Details PKC alpha

Product Details anti-PKC alpha Antibody Application Details Handling Images back to top
Antigen
Alternative Name PRKCA (PKCa Antibody Abstract)
Background Activation of PKC plays a crucial role in transducing signals for a variety of cellularresponses, including tumor proliferation (1). PKC isoforms have been classified intothree groups: classical, novel, and atypical. Members of these three PKC groups containan autoinhibitory domain that binds to the substrate binding site in the catalytic domain,preventing its activation in the absence of cofactors or activators (2,3). The activity ofPKC is under the control of three distinct phosphorylation events, including that of theThr641 autophosphorylation site (4). Alternate Names: anti-AAG6 antibody, anti-MGC129900 antibody, anti-MGC129901 antibody, anti-PICK 1antibody, anti-PKC A antibody, anti-PKC alpha antibody, anti-PKCA antibody,anti-PRKACA antibody, anti-PRKC A antibody, anti-PRKCA antibody, anti-ProteinKinase C alpha antibody, anti-Protein kinase C alpha type antibody.
Gene Symbol: PRKCA
Gene ID 5578, 18750, 25636
Pathways WNT Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-PKC alpha Antibody Target Details PKC alpha Handling Images back to top
Application Notes Recommended dilutions: Flow Cytometry 1:50, Immunocytochemistry/Immunofluorescence 1:250-500, Immunohistochemistry 1:100-250, Immunohistochemistry-Paraffin 1:100-250, Immunoprecipitation 1:30, Western Blot 1:2000
Protocol Protocol specific for PKC alpha Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-PKC alpha antibody (Protein Kinase C, alpha) (N-Term) (ABIN189675) Western blot analysis on K562 cell lysate using anti- PKC? RabMAb (cat. # 110-57353),...
Immunohistochemistry (IHC) image for anti-PKC alpha antibody (Protein Kinase C, alpha) (N-Term) (ABIN189675) Immunohistochemical analysis of paraffin-embedded human carcinoma of thyroid gland us...
Immunofluorescence (IF) image for anti-PKC alpha antibody (Protein Kinase C, alpha) (N-Term) (ABIN189675) Immunofluorescent staining of HeLa cells using anti-PKC? RabMAb (cat. #110-57353).