|Antigen||Protein Kinase C, beta (PRKCB) Antibodies|
|Epitope||Internal Region Alternatives|
|Reactivity||Cow (Bovine), Human, Monkey, Mouse (Murine), Rabbit, Rat (Rattus) Alternatives|
|Conjugate||This PKC beta antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|4 references available|
|Supplier||Log in to see|
Product Details anti-PKC beta AntibodyTarget Details PKC beta Application Details Handling References for anti-PKC beta antibody (ABIN401431) Images
|Specificity||This antibody is specific for PKC beta protein.|
Species reactivity (expected):Rabbit, Bovine, Rat, Mouse.
Species reactivity (tested):Human, Monkey.
|Immunogen||Synthetic peptide corresponding to an internal region of human Protein kinase C beta|
Target Details PKC betaProduct Details anti-PKC beta Antibody Application Details Handling References for anti-PKC beta antibody (ABIN401431) Images back to top
|Alternative Name||PRKCB (PRKCB Antibody Abstract)|
|Background||Protein kinase C (PKC) beta is implicated as a potential important factor in cancer cell proliferation and angiogenesis. PKC is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported.Synonyms: PKC beta, PKC-B, PKC-beta, PKCB, PRKCB1, Protein kinase C beta type|
|Pathways||WNT Signaling, TCR Signaling, Thyroid Hormone Synthesis, Nuclear Hormone Receptor Binding, Chromatin Binding, Myometrial Relaxation and Contraction, VEGF Signaling, Unfolded protein response|
Application DetailsProduct Details anti-PKC beta Antibody Target Details PKC beta Handling References for anti-PKC beta antibody (ABIN401431) Images back to top
ELISA: 1/75,000 - 1/80,000. Western Blot: 1/1,000 - 1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PKC beta Antibody Target Details PKC beta Application Details References for anti-PKC beta antibody (ABIN401431) Images back to top
|Concentration||1.0 mg/mL (by UV absorbance at 280 nm)|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Az|
|Precaution of Use|
|Handling Advice||Avoid repeated freezing and thawing.|
References for anti-PKC beta antibody (ABIN401431)Product Details anti-PKC beta Antibody Target Details PKC beta Application Details Handling Images back to top
Chen, Petrelli, Olesiak, Wilson, Labello, Pankiewicz: "Bis(sulfonamide) isosters of mycophenolic adenine dinucleotide analogues: inhibition of inosine monophosphate dehydrogenase." in: Bioorganic & medicinal chemistry, Vol. 16, Issue 15, pp. 7462-9, 2008
Koivunen, Aaltonen, Koskela, Lehenkari, Laato, Peltonen: "Protein kinase C alpha/beta inhibitor Go6976 promotes formation of cell junctions and inhibits invasion of urinary bladder carcinoma cells." in: Cancer research, Vol. 64, Issue 16, pp. 5693-701, 2004
Zhang, Wang, Petrin, Bishop, Bond: "Characterization of site-specific mutants altered at protein kinase C beta 1 isozyme autophosphorylation sites." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 13, pp. 6130-4, 1993
Flint, Paladini, Koshland: "Autophosphorylation of protein kinase C at three separated regions of its primary sequence." in: Science (New York, N.Y.), Vol. 249, Issue 4967, pp. 408-11, 1990
ImagesProduct Details anti-PKC beta Antibody Target Details PKC beta Application Details Handling References for anti-PKC beta antibody (ABIN401431) back to top