We are preparing the requested document. Please wait, this may take a while...!
|Antigen||Transcription Factor 7-Like 1 (T-Cell Specific, HMG-Box) (TCF7L1) Antibodies|
|Epitope||AA 35-84 Alternatives|
|Reactivity||Cow (Bovine), Guinea Pig, Human, Mouse (Murine), Rabbit, Rat (Rattus) Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-TCF7L1 AntibodyTarget Details TCF7L1 Application Details Handling Images
|Predicted Reactivity||Percent identity by BLAST analysis: Human, Mouse, Guinea pig (100%) Rabbit (85%).|
Synthetic peptide located between aa35-84 of human TCF7L1 (Q9HCS4, NP_112573). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Galago, Marmoset, Mouse, Rat, Bovine, Guinea pig (100%), Monkey, Elephant, Opossum (92%), Rabbit (85%).
Type of Immunogen: Synthetic peptide
Target Details TCF7L1Product Details anti-TCF7L1 Antibody Application Details Handling Images back to top
|Alternative Name||TCF7L1 (TCF7L1 Antibody Abstract)|
Name/Gene ID: TCF7L1
Synonyms: TCF7L1, TCF-3, Transcription factor 7-like 1, HMG box transcription factor 3
|Research Area||Chromatin and Nuclear Signaling, Chromatin Binding Proteins, Transcription Factors, Wnt, Wnt Signaling|
|Pathways||WNT Signaling, Stem Cell Maintenance|
Application DetailsProduct Details anti-TCF7L1 Antibody Target Details TCF7L1 Handling Images back to top
Approved: IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.
|Restrictions||For Research Use only|
HandlingProduct Details anti-TCF7L1 Antibody Target Details TCF7L1 Application Details Images back to top
|Buffer||Lyophilized from PBS with 2 % sucrose|
|Handling Advice||Avoid repeat freeze-thaw cycles.|
|Storage||4 °C,-20 °C|
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
ImagesProduct Details anti-TCF7L1 Antibody Target Details TCF7L1 Application Details Handling back to top