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anti-Human WNT3 antibody for ELISA

Recommended WNT3 Antibody (supplied by: Log in to see )

Antigen
Wingless-Type MMTV Integration Site Family, Member 3 (WNT3) Antibodies
  • Ci-Wnt3
  • Int-4
  • INT4
  • int4
  • wnt-3
  • Wnt-3
  • wnt3
  • WNT3
  • wnt3-A
  • wnt3l
  • Xwnt-3
  • Xwnt3
Epitope
Internal Region
28
12
10
10
8
3
2
1
1
1
Reactivity
Human, Mouse (Murine)
73
12
9
6
1
1
1
1
Host
Goat
35
19
19
Clonality
Polyclonal
Conjugate
This WNT3 antibody is un-conjugated
3
3
3
3
3
3
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
66
34
18
11
11
8
3
2
1
Supplier
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Catalog No. ABIN185634
€ 303.05
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0012099971 ABIN461935 IHC (p) ELISA WB Goat Internal Region Log in to see Polyclonal
0.0012099971 ABIN251471 ICC IF IHC (p) IHC ELISA WB Goat IgG Internal Region Log in to see Polyclonal
0.0012099971 ABIN2466453 ICC IF IHC ELISA WB Goat Internal Region Log in to see Polyclonal
0.0012099971 ABIN1948747 ELISA WB Alkaline Phosphatase (AP) Rabbit IgG Internal Region, AA 154-184 Log in to see Polyclonal
0.0012099971 ABIN1948749 ELISA WB Biotin Rabbit IgG Internal Region, AA 154-184 Log in to see Polyclonal
0.0012099971 ABIN1948748 ELISA WB APC Rabbit IgG Internal Region, AA 154-184 Log in to see Polyclonal
0.0012099971 ABIN1948750 ELISA WB FITC Rabbit IgG Internal Region, AA 154-184 Log in to see Polyclonal
0.0012099971 ABIN1948751 ELISA WB PE Rabbit IgG Internal Region, AA 154-184 Log in to see Polyclonal
0.0012099971 ABIN1948752 ELISA WB HRP Rabbit IgG Internal Region, AA 154-184 Log in to see Polyclonal
0.0012099971 ABIN2477143 IF IHC (p) ELISA WB Goat IgG Log in to see Polyclonal 1
0.0012099971 ABIN1740828 IHC (p) ELISA WB Goat Internal Region Log in to see Polyclonal
0.0012099971 ABIN2570915 ELISA Rabbit IgG AA 154-184 Log in to see Polyclonal
0.0012099971 ABIN2387238 ELISA WB Rabbit IgG AA 155-184, Center, Internal Region Log in to see Polyclonal
0.0012099971 ABIN2387240 ELISA WB HRP Rabbit IgG AA 155-184, Center, Internal Region Log in to see Polyclonal
0.0012099971 ABIN2387244 ELISA WB APC Rabbit IgG AA 155-184, Center, Internal Region Log in to see Polyclonal
0.0012099971 ABIN2387251 IC IF IHC ELISA WB Alkaline Phosphatase (AP) Goat Internal Region Log in to see Polyclonal
0.0012099971 ABIN2387253 IC IF IHC ELISA WB Biotin Goat Internal Region Log in to see Polyclonal
0.0012099971 ABIN2387256 IC IF IHC ELISA WB PE Goat Internal Region Log in to see Polyclonal
0.0012099971 ABIN2387239 ELISA WB FITC Rabbit IgG AA 155-184, Center, Internal Region Log in to see Polyclonal
0.0012099971 ABIN2387241 ELISA WB PE Rabbit IgG AA 155-184, Center, Internal Region Log in to see Polyclonal

Top referenced anti-WNT3 antibody for ELISA

Similar anti-WNT3 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 7 Antibodies 36 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 2 Antibodies 4 Antibodies
Immunofluorescence (IF) 4 Antibodies 12 Antibodies
Immunohistochemistry (IHC) 4 Antibodies 20 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 4 Antibodies 12 Antibodies
Western Blotting (WB) 11 Antibodies 68 Antibodies
Immunochromatography (IC) 8 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies

General

Antigen Wingless-Type MMTV Integration Site Family, Member 3 (WNT3) Antibodies
Epitope Internal Region
(28), (12), (10), (10), (8), (3), (2), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(73), (12), (9), (6), (1), (1), (1), (1)
Host Goat
(35), (19), (19)
Clonality
Polyclonal
Conjugate This WNT3 antibody is un-conjugated
(3), (3), (3), (3), (3), (3)
Methode Type Peptide ELISA
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(66), (34), (18), (11), (11), (8), (3), (2), (1)
Pubmed 4 references available
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Product Details anti-WNT3 Antibody

Target Details WNT3 Application Details Handling References for anti-WNT3 Antibody (ABIN185634) Images
Blocking Peptide Blocking peptide for this product available: ABIN369133
Cross-Reactivity Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen Peptide with sequence CGRGHNTRTEKRKEK, from the internal region of the protein sequence according to NP_110380.1.

Target Details WNT3

Product Details anti-WNT3 Antibody Application Details Handling References for anti-WNT3 Antibody (ABIN185634) Images back to top
Antigen
Alternative Name WNT3 (WNT3 Antibody Abstract)
Background Synonyms: WNT3, wingless-type MMTV integration site family, member 3, INT4, MGC131950, MGC138321, MGC138323, WNT-3 proto-oncogene protein precursor
Gene ID 7473, 22415, 24882
NCBI Accession NP_110380
Pathways WNT Signaling

Application Details

Product Details anti-WNT3 Antibody Target Details WNT3 Handling References for anti-WNT3 Antibody (ABIN185634) Images back to top
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 40 kDa band observed in lysates of cell line HEK293 (calculated MW of 39.6 kDa according to NP_110380.1). Recommended concentration: 1-3 µg/mL.
IHC: In paraffin embedded Human Breast shows strong fibrous staining in myoepithelial cells. Data obtained from a previous batch (different goat). Recommended concentration: 3-5 µg/mL. Immunocytochemsitry/Immunofluorescence This antibody has been successfully used in ICC/IF for mouse hippocampal primary astrocytes as described in the following paper: Okamoto et al, FASEB J. 2011 Jul 11, PMID: 21746862.
Restrictions For Research Use only

Handling

Product Details anti-WNT3 Antibody Target Details WNT3 Application Details References for anti-WNT3 Antibody (ABIN185634) Images back to top
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

References for anti-WNT3 Antibody (ABIN185634)

Product Details anti-WNT3 Antibody Target Details WNT3 Application Details Handling Images back to top
Product cited in:

Fujimaki, Hidaka, Asashima, Takemasa, Kuwabara: "Wnt protein-mediated satellite cell conversion in adult and aged mice following voluntary wheel running." in: The Journal of biological chemistry, Vol. 289, Issue 11, pp. 7399-412, 2014

Okamoto, Inoue, Iwamura, Terashima, Soya, Asashima, Kuwabara: "Reduction in paracrine Wnt3 factors during aging causes impaired adult neurogenesis." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 10, pp. 3570-82, 2011

Background publications

Schmitt, Shi, Wolf, Lu, King, Zou: "Wnt-Ryk signalling mediates medial-lateral retinotectal topographic mapping." in: Nature, Vol. 439, Issue 7072, pp. 31-7, 2006

Lie, Colamarino, Song, Desire, Mira, Consiglio, Lein, Jessberger, Lansford, Dearie, Gage: "Wnt signalling regulates adult hippocampal neurogenesis." in: Nature, Vol. 437, Issue 7063, pp. 1370-5, 2005

Images

Product Details anti-WNT3 Antibody Target Details WNT3 Application Details Handling References for anti-WNT3 Antibody (ABIN185634) back to top
Supplier Images
 image for anti-WNT3 antibody (Wingless-Type MMTV Integration Site Family, Member 3) (Internal Region) (ABIN185634) ABIN185634 (1 ug/ml) staining of Human Brain lysate (35 ug protein in RIPA buffer). ...
 image for anti-WNT3 antibody (Wingless-Type MMTV Integration Site Family, Member 3) (Internal Region) (ABIN185634) ABIN185634 (1 µg/mL) staining of lysate of cell line HEK293 (35 µg protein in RIPA bu...
 image for anti-WNT3 antibody (Wingless-Type MMTV Integration Site Family, Member 3) (Internal Region) (ABIN185634) ABIN185634 (3.8 µg/mL) staining of paraffin embedded Human Breast. Steamed antigen re...
 image for anti-WNT3 antibody (Wingless-Type MMTV Integration Site Family, Member 3) (Internal Region) (ABIN185634) ABIN185634 (2 µg/mL) staining of paraffin embedded Human Breast. Steamed antigen retr...