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|Application / Reactivity||Mouse (Murine)||Human|
|ELISA||7 Antibodies||36 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies||2 Antibodies|
|Immunocytochemistry (ICC)||2 Antibodies||4 Antibodies|
|Immunofluorescence (IF)||4 Antibodies||12 Antibodies|
|Immunohistochemistry (IHC)||4 Antibodies||19 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||4 Antibodies||12 Antibodies|
|Western Blotting (WB)||11 Antibodies||64 Antibodies|
|Immunochromatography (IC)||8 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Antigen||Wingless-Type MMTV Integration Site Family, Member 3 (WNT3) Antibodies|
|Epitope||Internal Region Alternatives|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This WNT3 antibody is un-conjugated Alternatives|
|Methode Type||Peptide ELISA|
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
|4 references available|
|Supplier||Log in to see|
Product Details anti-WNT3 AntibodyTarget Details WNT3 Application Details Handling References for anti-WNT3 Antibody (ABIN185634) Images
|Blocking Peptide||Blocking peptide for this product available: ABIN369133|
|Cross-Reactivity||Rat (Rattus), Dog (Canine)|
|Purification||Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.|
|Immunogen||Peptide with sequence CGRGHNTRTEKRKEK, from the internal region of the protein sequence according to NP_110380.1.|
Target Details WNT3Product Details anti-WNT3 Antibody Application Details Handling References for anti-WNT3 Antibody (ABIN185634) Images back to top
|Alternative Name||WNT3 (WNT3 Antibody Abstract)|
|Background||Synonyms: WNT3, wingless-type MMTV integration site family, member 3, INT4, MGC131950, MGC138321, MGC138323, WNT-3 proto-oncogene protein precursor|
|Gene ID||7473, 22415, 24882|
Application DetailsProduct Details anti-WNT3 Antibody Target Details WNT3 Handling References for anti-WNT3 Antibody (ABIN185634) Images back to top
ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 40 kDa band observed in lysates of cell line HEK293 (calculated MW of 39.6 kDa according to NP_110380.1). Recommended concentration: 1-3 µg/mL.
IHC: In paraffin embedded Human Breast shows strong fibrous staining in myoepithelial cells. Data obtained from a previous batch (different goat). Recommended concentration: 3-5 µg/mL. Immunocytochemsitry/Immunofluorescence This antibody has been successfully used in ICC/IF for mouse hippocampal primary astrocytes as described in the following paper: Okamoto et al, FASEB J. 2011 Jul 11, PMID: 21746862.
|Restrictions||For Research Use only|
HandlingProduct Details anti-WNT3 Antibody Target Details WNT3 Application Details References for anti-WNT3 Antibody (ABIN185634) Images back to top
|Buffer||Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Minimize freezing and thawing.|
|Storage Comment||Aliquot and store at -20 °C.|
References for anti-WNT3 Antibody (ABIN185634)Product Details anti-WNT3 Antibody Target Details WNT3 Application Details Handling Images back to top
|Product cited in:||
Fujimaki, Hidaka, Asashima et al.: "Wnt protein-mediated satellite cell conversion in adult and aged mice following voluntary wheel running." in: The Journal of biological chemistry, Vol. 289, Issue 11, pp. 7399-412, 2014 (PubMed).
Okamoto, Inoue, Iwamura et al.: "Reduction in paracrine Wnt3 factors during aging causes impaired adult neurogenesis." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 10, pp. 3570-82, 2011 (PubMed).
Schmitt, Shi, Wolf et al.: "Wnt-Ryk signalling mediates medial-lateral retinotectal topographic mapping." in: Nature, Vol. 439, Issue 7072, pp. 31-7, 2006 (PubMed).
Lie, Colamarino, Song et al.: "Wnt signalling regulates adult hippocampal neurogenesis." in: Nature, Vol. 437, Issue 7063, pp. 1370-5, 2005 (PubMed).
ImagesProduct Details anti-WNT3 Antibody Target Details WNT3 Application Details Handling References for anti-WNT3 Antibody (ABIN185634) back to top