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anti-Chicken WNT5A antibody for Western Blotting

Recommended WNT5A Antibody (supplied by: Log in to see )

Antigen
Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) Antibodies
  • 8030457G12Rik
  • hWNT5A
  • wnt-5A
  • Wnt-5a
  • WNT5A
  • wnt5a-B
Epitope
Middle Region
15
14
13
10
6
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Xenopus laevis
86
34
23
13
1
Host
Rabbit
65
31
1
1
Clonality
Polyclonal
Conjugate
This WNT5A antibody is un-conjugated
5
4
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
75
51
49
19
16
11
10
3
1
1
1
1
1
Supplier
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Catalog No. ABIN2779470
$ 289.00
Plus shipping costs $45.00

Top referenced anti-WNT5A antibody for Western Blotting

Similar anti-WNT5A Antibodies

Application / Reactivity Human Chicken Cow (Bovine) Mouse (Murine) Rabbit Rat (Rattus) Xenopus laevis
Western Blotting (WB) 70 Antibodies 1 Antibodies 1 Antibodies 20 Antibodies 1 Antibodies 14 Antibodies 1 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 18 Antibodies 5 Antibodies 5 Antibodies
Immunohistochemistry (IHC) 48 Antibodies 1 Antibodies 1 Antibodies 11 Antibodies 1 Antibodies 7 Antibodies 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies

General

Antigen Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) Antibodies
Epitope Middle Region
(15), (14), (13), (10), (6), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Xenopus laevis
(86), (34), (23), (13), (1)
Host Rabbit
(65), (31), (1), (1)
Clonality
Polyclonal
Conjugate This WNT5A antibody is un-conjugated
(5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(75), (51), (49), (19), (16), (11), (10), (3), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-WNT5A Antibody

Target Details WNT5A Application Details Handling References for anti-WNT5A Antibody (ABIN2779470) Images
Blocking Peptide Blocking peptide for this product available: ABIN986222
Sequence GGCGDNIDYG YRFAKEFVDA RERERIHAKG SYESARILMN LHNNEAGRRT
Predicted Reactivity Bovine : 100 %, Dog : 100 %, Guinea pig : 100 %, Human : 100 %, Mouse : 100 %, Rabbit : 100 %, Rat : 100 %, African clawed frog : 90 %, Chicken : 90 %
Characteristics This is a rabbit polyclonal antibody against WNT5A. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human WNT5A

Target Details WNT5A

Product Details anti-WNT5A Antibody Application Details Handling References for anti-WNT5A Antibody (ABIN2779470) Images back to top
Antigen
Alternative Name WNT5A (WNT5A Antibody Abstract)
Background The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT5A is a member of the WNT protein family. It shows 98 %, 98 % and 88 % amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction.The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98 %, 98 % and 88 % amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction.
Molecular Weight 42 kDa
Gene ID 7474
NCBI Accession NP_003383, NM_003392
UniProt P41221
Pathways WNT Signaling

Application Details

Product Details anti-WNT5A Antibody Target Details WNT5A Handling References for anti-WNT5A Antibody (ABIN2779470) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 380 AA

Restrictions For Research Use only

Handling

Product Details anti-WNT5A Antibody Target Details WNT5A Application Details References for anti-WNT5A Antibody (ABIN2779470) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-WNT5A Antibody (ABIN2779470)

Product Details anti-WNT5A Antibody Target Details WNT5A Application Details Handling Images back to top
Background publications

Witze, Litman, Argast, Moon, Ahn: "Wnt5a control of cell polarity and directional movement by polarized redistribution of adhesion receptors." in: Science (New York, N.Y.), Vol. 320, Issue 5874, pp. 365-9, 2008

Images

Product Details anti-WNT5A Antibody Target Details WNT5A Application Details Handling References for anti-WNT5A Antibody (ABIN2779470) back to top
Supplier Images
Western Blotting (WB) image for anti-WNT5A antibody (Wingless-Type MMTV Integration Site Family, Member 5A) (Middle Region) (ABIN2779470) anti-Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) (Middle Region) antibody
Immunohistochemistry (IHC) image for anti-WNT5A antibody (Wingless-Type MMTV Integration Site Family, Member 5A) (Middle Region) (ABIN2779470) anti-Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) (Middle Region) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-WNT5A antibody (Wingless-Type MMTV Integration Site Family, Member 5A) (Middle Region) (ABIN2779470) anti-Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) (Middle Region) antibody (Image 3)