anti-Mouse (Murine) WNT5A antibody for Western Blotting

Recommended WNT5A Antibody (supplied by: Log in to see )

Antigen
Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) Antibodies
  • hWNT5A
  • wnt-5A
  • wnt5a-B
  • WNT5A
  • 8030457G12Rik
  • Wnt-5a
  • wingless-type MMTV integration site family, member 5A
  • wingless-type MMTV integration site family, member 5a
  • wingless-related MMTV integration site 5A
  • Wnt signaling ligand
  • WNT-5A protein
  • WNT5A
  • Wnt5a
  • wnt5a
  • LOC578814
  • wnt5
  • WNT-5A
Epitope
AA 330-380
18
14
14
11
7
5
5
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
137
45
33
17
5
3
3
2
2
1
1
1
1
1
Host
Rabbit
89
56
2
1
Clonality
Polyclonal
Conjugate
This WNT5A antibody is un-conjugated
7
6
5
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
111
62
55
46
17
12
11
4
2
1
1
1
1
1
Options
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Catalog No. ABIN675758
$ 307.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1474795 ABIN1078673 ICC IHC IP WB Rabbit IgG AA 62-380 Log in to see Polyclonal
3.1474795 ABIN1171776 IHC ELISA WB Biotin Rabbit IgG Log in to see Polyclonal
3.1474795 ABIN1875353 IHC WB Rabbit IgG Log in to see Polyclonal
3.1474795 ABIN3023003 IHC WB Rabbit IgG Log in to see Polyclonal
3.1474795 ABIN635529 WB Rabbit Middle Region Log in to see Polyclonal
3.1474795 ABIN3029635 IHC (p) WB Rabbit IgG AA 184-303 Log in to see Polyclonal
3.1474795 ABIN5534831 FACS IHC (p) WB Rabbit Ig Fraction AA 185-213 Log in to see Polyclonal
3.1474795 ABIN5545279 WB Rabbit IgG Log in to see Polyclonal
1 ABIN2779470 IHC WB Rabbit Middle Region Log in to see Polyclonal 1
1 ABIN207746 IHC (p) WB Rabbit IgG AA 190-240 Log in to see Polyclonal
1 ABIN574490 IHC (p) WB Rabbit IgG AA 180-229 Log in to see Polyclonal
1 ABIN675767 IHC (p) WB HRP Rabbit IgG AA 330-380 Log in to see Polyclonal
1 ABIN4366188 IHC IHC (p) WB Rabbit Log in to see Polyclonal 9
1 ABIN675760 IHC (p) WB Biotin Rabbit IgG AA 330-380 Log in to see Polyclonal
1 ABIN602565 IHC ELISA WB Rat IgG2a AA 38-380 Log in to see
1 ABIN2387017 IC IHC ELISA WB Rabbit IgG AA 62-381 Log in to see Polyclonal
1 ABIN2629874 WB Rabbit IgG AA 184-303 Log in to see Polyclonal
1 ABIN222852 ELISA WB Rabbit Leu270 Log in to see Polyclonal
1 ABIN2570929 ELISA WB Biotin Rabbit IgG AA 62-381 Log in to see Polyclonal
1 ABIN2570930 ELISA WB FITC Rabbit IgG AA 62-381 Log in to see Polyclonal

General

Antigen Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) Antibodies
Epitope AA 330-380
(18), (14), (14), (11), (7), (5), (5), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(137), (45), (33), (17), (5), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(89), (56), (2), (1)
Clonality
Polyclonal
Conjugate This WNT5A antibody is un-conjugated
(7), (6), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(111), (62), (55), (46), (17), (12), (11), (4), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-WNT5A Antibody

Target Details WNT5A Application Details Handling References for anti-WNT5A antibody (ABIN675758) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human WNT5A
Isotype IgG

Target Details WNT5A

Product Details anti-WNT5A Antibody Application Details Handling References for anti-WNT5A antibody (ABIN675758) Images back to top
Antigen
Alternative Name Wnt5a (WNT5A Antibody Abstract)
Background

Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes.

Subcellular location: Secreted

Synonyms: hWNT5A, Protein Wnt-5a, WNT5A

Gene ID 7474
UniProt P41221
Pathways WNT Signaling, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Cell Size, Tube Formation

Application Details

Product Details anti-WNT5A Antibody Target Details WNT5A Handling References for anti-WNT5A antibody (ABIN675758) Images back to top
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only

Handling

Product Details anti-WNT5A Antibody Target Details WNT5A Application Details References for anti-WNT5A antibody (ABIN675758) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-WNT5A antibody (ABIN675758)

Product Details anti-WNT5A Antibody Target Details WNT5A Application Details Handling Images back to top
Product cited in:

Saha, Aranda, Hayakawa, Bhanja, Atay, Brodin, Li, Asfaha, Liu, Tailor, Zhang, Godwin, Tome, Wang, Guha, Pollard: "Macrophage-derived extracellular vesicle-packaged WNTs rescue intestinal stem cells and enhance survival after radiation injury." in: Nature communications, Vol. 7, pp. 13096, 2016

Król, Mucha, Majchrzak, Homa, Bulkowska, Majewska, Gajewska, Pietrzak, Perszko, Romanowska, Paw?owski, Manuali, Hellmen, Motyl: "Macrophages mediate a switch between canonical and non-canonical Wnt pathways in canine mammary tumors." in: PLoS ONE, Vol. 9, Issue 1, pp. e83995, 2014

Images

Product Details anti-WNT5A Antibody Target Details WNT5A Application Details Handling References for anti-WNT5A antibody (ABIN675758) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) (AA 330-380) antibody (ABIN675758) Frozen mouse brain sections labeled with Anti-WNT5A Polyclonal Antibody, Unconjugated...
Immunohistochemistry (IHC) image for anti-Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) (AA 330-380) antibody (ABIN675758) Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-WNT5A Pol...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) (AA 330-380) antibody (ABIN675758) Image kindly submitted by Dr. Magdalena Krol. Formalin-fixed and paraffin embedded ca...
Western Blotting (WB) image for anti-Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) (AA 330-380) antibody (ABIN675758) Image kindly provided by Dr. Magdalena Krol. Control tumor cells, tumor cells grown i...
Western Blotting (WB) image for anti-Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) (AA 330-380) antibody (ABIN675758) Mouse ovary lysates probed with WNT5A Polyclonal Antibody, unconjugated at 1:300 ove...
Western Blotting (WB) image for anti-Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) (AA 330-380) antibody (ABIN675758) Mouse uterus lysates probed with WNT5A Polyclonal Antibody, unconjugated at 1:300 ov...