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ACA-IgM ELISA Kit

ACA-IgM Reactivity: Mouse Colorimetric Sandwich ELISA 3.125 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Catalog No. ABIN5522880
  • Target
    ACA-IgM (Anti-Cardiolipin Antibody IgM (ACA-IgM))
    Reactivity
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    3.125 ng/mL - 200 ng/mL
    Minimum Detection Limit
    3.125 ng/mL
    Application
    ELISA
    Purpose
    For quantitative detection of ACA-IgM in serum, plasma, tissue homogenates.
    Sample Type
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of ACA-IgM. No significant cross- reactivity or interference between ACA-IgM and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between ACA-IgM and all the analogues, therefore, cross reaction may still exist.
    Sensitivity
    1.875 ng/mL
    Components
    - ELISA Microplate
    - Lyophilized Standard
    - Sample / Standard Dilution Buffer
    - Biotin-labeled Antibody (Concentrated)
    - Antibody Dilution Buffer
    - HRP-Streptavidin Conjugate(SABC)
    - SABC Dilution Buffer
    - TMB Substrate
    - Stop Solution
    - Wash Buffer (25X)
    - Plate Sealer
    - Product Description
    Material not included
    - Microplate reader (wavelength: 450nm)
    - 37 °C incubator
    - Automated plate washer
    - Precision single and multi-channel pipette and disposable tips
    - Clean tubes and Eppendorf tubes
    - Deionized or distilled water
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Comment

    Sandwich ELISA, Double Antigen

    Sample Volume
    100 μL
    Plate
    Pre-coated
    Restrictions
    For Research Use only
  • Storage
    4 °C
    Storage Comment
    1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
    2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.
    Expiry Date
    6 months
  • Target
    ACA-IgM (Anti-Cardiolipin Antibody IgM (ACA-IgM))
    Alternative Name
    Anti-Cardiolipin Antibody IgM
    Target Type
    Antibody
    Background
    ACA-IgM/anti-Cardiolipin Antibody IgM
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