TRAIL and Receptor Detection Set
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- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
- Purification
- Antibodies are supplied as affinity chromatography purified IgG.
- Components
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TRAIL Antibody, (50 μg)
DR4 Antibody, (50 μg)
DR5 Antibody, (50 μg)
DcR1 Antibody, (50 μg)
DcR2 Antibody, (50 μg)
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- Application Notes
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These polyclonal antibodies can be used for detection of TRAIL and its receptors by Western blot at 2 μg/mL to 1 μg/Ml, Immunohistochemistry, and immunoflourescence.
Optimal dilutions for each application to be determined by the researcher. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS containing 0.02 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Stable at 4 °C for three months, store at -20 °C for up to one year.
- Expiry Date
- 12 months
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- Background
- Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organism. It is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family which is expressed in a variety of human tissues. Similar to TNF and Fas ligand, TRAIL induces apoptosis and NF-κB activation in many tissues and cells. Its receptors include the death domain containing proteins DR4 and DR5, both of which mediate TRAIL induced cell death. Two decoy receptors, DcR1 and DcR2, have also been identified. Both of these proteins contain an extracellular TRAIL binding domain but lack intracellular signaling domains. Overexpression of either DcR1 or DcR2 attenuates TRAIL-induced apoptosis.
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