I kappa B Kinase Detection Set
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- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
- Purification
- Antibodies are supplied as affinity chromatography purified IgG.
- Components
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IKK±, Antibody, (50 μg)
IKK², Antibody, (50 μg)
IKK³ Antibody, (50 μg)
IKK? Antibody, (50 μg)
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- Application Notes
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These polyclonal antibodies can be used for detection of IKK±, IKK², IKK³ and IKK? by immunoblot at 0.5 - 2 μg/mL, and by immuncytochemistry (IKK±, IKK², and IKK³) or immunohistochemistry (IKK?) at 1 - 10 μg/mL, and Immunoflourescence.
Optimal dilutions for each application to be determined by the researcher. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS containing 0.02 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Stable at 4 °C for three months, store at -20 °C for up to one year.
- Expiry Date
- 12 months
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- Background
- Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFα, and bacteria product LPS. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. IκB kinase (IKK), which phosphorylates IκB and mediates IκB degradation and NF-κB activation, was recently identified by several laboratories. IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKα, and IKKβ,). IKKα, and IKKβ, interact with each other and both are essential for the NF-κB activation. IKKα, specifically phosphorylates IκB-α, while IKKβ, phosphorylates both IκB-α, and IκB-β,. Another molecule in the IKK complex termed IKKγ (also known as NEMO) interacts with IKKβ, and is required for the activation of IKK complex and NF-κB by LPS, PMA, TNF, and IL-1 stimulation. IKKγ was also shown to bind to RIP and NIK and to mediate TNF-induced NF-κB activation. IKKε is required for the activation of NF-κB by PMA and T cell receptors but not by TNFα, and IL-1. IKKε message is expressed in a variety of tissues and is inducible by TNFα, IL-1, and LPS.
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