ANXA2P2 Protein (AA 2-339) (His tag)
Crystallography grade
ANXA2P2
Origin: Human
Host: Escherichia coli (E. coli)
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Crys, ELISA, SDS, WB
2 images
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- Target
- ANXA2P2 (Annexin A2 Pseudogene 2 (ANXA2P2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-339
- Origin
- Human
- Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This ANXA2P2 protein is labelled with His tag.
- Application
- Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequence
- STVHEILCKL SLEGDHSTPP SAYGSVKAYT NFDAERDALN IETAIKTKGV DEVTIVNIVT NRDNAQRQDI VFSYQRRTKK ELASALKSAL SGHLETVILG LLKTPAQYDA SELKASMKGL GTDEDSLIEI ICSRTNQELQ EINRVYKEMY KTDLEKDIIS DTSGDFRKLM VALAKGRRAE DGSVIDYELI DQDAQDLYDA GVKRKGTDVP KWISIMTERS VPHLQKVFDR YKSYSPYDML ESIRKEVKGD LENAFLNLVQ RIQNKPLYFA DQLYDSMKGK GTRDKVLIRI MVSRSEVDML KIRSEFKRKY GKSLYYYIQQ DTKGDYQKAL LYLCGGDD
- Characteristics
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This made-to-order protein has already been successfully produced. Please let us know if you are interested in purchasing a smaller amount of this protein. We will check our stock and make you a customized quote in case we can provide this protein in a smaller amount.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
-
Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- Grade
- Crystallography grade
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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N-terminal His-tag
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 50 mM Hepes, pH 7.8; 300 mM NaCl, 5 % glycerin
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- ANXA2P2 (Annexin A2 Pseudogene 2 (ANXA2P2))
- Alternative Name
- ANXA2P2
- Synonyms
- ANX2L2 Protein, ANX2P2 Protein, LPC2B Protein, annexin A2 pseudogene 2 Protein, ANXA2P2 Protein
- Background
- Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. {ECO:0000250}.
- Molecular Weight
- 39.5 kDa Including tag.
- UniProt
- A6NMY6
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