Methylprednisolone antibody
ELISA, DB, IHC, WB
Host: Sheep
Monoclonal
unconjugated
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- Target See all Methylprednisolone products
- Methylprednisolone
- Reactivity
- Please inquire
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Host
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Sheep
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Clonality
- Monoclonal
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Conjugate
- This Methylprednisolone antibody is un-conjugated
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Application
- ELISA, Dot Blot (DB), Immunohistochemistry (IHC), Western Blotting (WB)
- Specificity
- ELISA microtitre plate based and expressed as % crossreactivity*. The antibody exhibited crossreactivity for the following compounds: Methylprednisolone 100% Prednisolone 23.90% Triamcinolone < 1.8% * crossreactivity profile may vary with tracer used
- Purification
- Protein A affinity chromatography
- Immunogen
- Methylprednisolone-BTG
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- Application Notes
- Working Concentration: 0.25 µg/mL (EIA)
- Restrictions
- For Research Use only
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- Concentration
- 4.396 mg/mL (U.V. abs at 280 nm)
- Buffer
- PBS at pH 7.3-7.9
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
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- Target
- Methylprednisolone
- Abstract
- Methylprednisolone Products
- Target Type
- Chemical
- Background
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Non-steroidal anti-inflammatory drugs, usually abbreviated to NSAIDs or NAIDs , are drugs with analgesic, antipyretic and, in higher doses, anti-inflammatory effects - they reduce pain, fever and inflammation. Most NSAIDs act as nonselective inhibitors of the enzyme cyclooxygenase, inhibiting both the cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) isoenzymes. Cyclooxygenase catalyzes the formation of prostaglandins and thromboxane from arachidonic acid. Prostaglandins are short-lived substances made from fatty acids that play a role in many body functions. They influence pain signals, regulation of inflammation, maintenance of tissues such as the lining of the stomach, blood clotting and stimulation of cells into action. In whole the NSAIDs prevent the prostaglandins from ever being synthesized, therefore reducing or eliminating the pain.
Method of Production: In vitro cell culture using standard DMEM media supplemented with foetal bovine serum of a USDA approved origin.
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