GFP antibody (HRP)

Catalog No. ABIN100089

Supplier Product No.: 600-103-215

This anti-GFP antibody is a Goat Polyclonal antibody detecting GFP in WB, ELISA and IHC. Suitable for Aequorea victoria. This Primary Antibody has been cited in 11+ publications.

Quick Overview for GFP antibody (HRP) (ABIN100089)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Goat

Clonality: Polyclonal

Conjugate: This GFP antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-GFP Antibody (HRP)

Supplier Product No.: 600-103-215

Supplier: Rockland

Purpose: GFP Antibody Peroxidase Conjugate

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Peroxidase and purified and partially purified Green Fluorescent Protein (Aequorea victoria).

Characteristics: Synonyms: goat anti-GFP Antibody peroxidase Conjugation, HRP conjugated goat anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish

Purification: Anti-GFP antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen:

Immunogen: Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500 - 1:2,000

Application Note: Polyclonal anti-GFP is designed to detect GFP and its variants. Anti-GFP Peroxidase conjugated antibody has been tested by ELISA to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP and by western blot. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with Rockland's monoclonal anti-GFP (600-301-215) using either form of the antibody as the capture or detection antibody. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP (code # S000-03). Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.

Western Blot Dilution: 1:2,000 - 1:5,000

ELISA Dilution: 1:10,000 - 1:50,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store GFP antibody at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-GFP antibody (HRP) (ABIN100089)

Hennigan, Fletcher, Guard, Ratner: "Proximity biotinylation identifies a set of conformation-specific interactions between Merlin and cell junction proteins." in: Science signaling, Vol. 12, Issue 578, (2020) (PubMed).

Lim, Chang, Huh: "Phosphoregulation of Rad51/Rad52 by CDK1 functions as a molecular switch for cell cycle-specific activation of homologous recombination." in: Science advances, Vol. 6, Issue 6, pp. eaay2669, (2020) (PubMed).

Bisgrove, Su, Yost: "Maternal Gdf3 is an obligatory cofactor in Nodal signaling for embryonic axis formation in zebrafish." in: eLife, Vol. 6, (2018) (PubMed).

Botto, Totonchy, Gustin, Moses: "Kaposi Sarcoma Herpesvirus Induces HO-1 during De Novo Infection of Endothelial Cells via Viral miRNA-Dependent and -Independent Mechanisms." in: mBio, Vol. 6, Issue 3, pp. e00668, (2016) (PubMed).

Fahrenkamp, de Leur, Küster, Chatain, Müller-Newen: "Src family kinases interfere with dimerization of STAT5A through a phosphotyrosine-SH2 domain interaction." in: Cell communication and signaling : CCS, Vol. 13, pp. 10, (2015) (PubMed).

Gustin, Bai, Moses, Douglas: "Ebola Virus Glycoprotein Promotes Enhanced Viral Egress by Preventing Ebola VP40 From Associating With the Host Restriction Factor BST2/Tetherin." in: The Journal of infectious diseases, Vol. 212 Suppl 2, pp. S181-90, (2015) (PubMed).

Bejarano, Yuste, Patel, Stout, Spray, Cuervo: "Connexins modulate autophagosome biogenesis." in: Nature cell biology, Vol. 16, Issue 5, pp. 401-14, (2014) (PubMed).

Sung, Lim, Yi, Chang, Yang, Lee, Huh: "Genome-wide bimolecular fluorescence complementation analysis of SUMO interactome in yeast." in: Genome research, Vol. 23, Issue 4, pp. 736-46, (2013) (PubMed).

Chatain, Ziegler, Fahrenkamp, Jost, Moriggl, Schmitz-Van de Leur, Müller-Newen: "Src family kinases mediate cytoplasmic retention of activated STAT5 in BCR-ABL-positive cells." in: Oncogene, (2012) (PubMed).

Ha, Sung, Huh: "Nsi1 plays a significant role in the silencing of ribosomal DNA in Saccharomyces cerevisiae." in: Nucleic acids research, Vol. 40, Issue 11, pp. 4892-903, (2012) (PubMed).

Guarino, Shepherd, Salguero, Hua, Deegan, Kearsey: "Cdt1 proteolysis is promoted by dual PIP degrons and is modulated by PCNA ubiquitylation." in: Nucleic acids research, Vol. 39, Issue 14, pp. 5978-90, (2011) (PubMed).

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP

Background: Background: HRP Anti-GFP is ideal for western blotting, ELISA and Immunohistochemistry. Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.

UniProt: P42212