Rabbit anti-Chicken IgG (Heavy & Light Chain) Antibody

Details for Product No. ABIN101028
Antigen
Epitope
Heavy & Light Chain
1569
134
100
56
34
33
25
6
6
6
6
5
4
4
4
3
2
1
1
1
Reactivity
Chicken
355
342
336
233
232
122
113
87
79
73
72
65
61
57
54
49
43
31
27
15
9
9
7
5
4
4
4
4
4
3
3
3
3
2
2
1
1
1
1
Host
Rabbit
846
798
335
169
93
84
23
22
13
5
4
4
4
3
1
1
1
1
Clonality
Polyclonal
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
Options
Immunogen

Immunogen: Chicken IgG whole molecule

Specificity IgG (H&L)
Characteristics Concentration Definition: by Refractometry
Purification This product was prepared from monospecific antiserum by a delipidation and defibrination.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, Chicken IgG and Chicken Serum.
Antigen
Background

Synonyms: rabbit anti-Chicken IgG Antibody, rabbit anti Chicken IgG

Background: Anti-Chicken IgG Antibody generated in rabbit detects chicken IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.

Application Notes

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Chicken IgG Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration 90 mg/mL
Buffer

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: None

Preservative Without preservative
Storage RT,4 °C,-20 °C
Expiry Date 12 months