N epsilon Hexanoyl-Keyhole Limpet Hemocyanin (HEL-KLH) antibody

Catalog No. ABIN1020378

Product Details

Target: N epsilon Hexanoyl-Keyhole Limpet Hemocyanin (HEL-KLH)

Reactivity: Keyhole Limpet

Host: Human, Rat

Clonality: Monoclonal

Application: Immunohistochemistry (IHC), ELISA

Specificity: Specific to Hexanoyl-Lys adducts.

Clone: 5F12

Isotype: IgG1 kappa

Application Details

Application Notes: Recommended antibody concentration is 2 µg/mL on paraformaldehyde fixed tissue.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Containing 10mM PBS(pH7.4), 5% sucrose, 1% BSA and 0.05% Procline 950. 100 µg/mL of IgG.

Preservative: ProClin

Storage: -20 °C

References

Sugiyama, Sun, Nishinohara, Fujita, Masuda, Ochi, Takeuchi: "Expressions of lipid oxidation markers, N(ε)-hexanoyl lysine and acrolein in cisplatin-induced nephrotoxicity in rats." in: The Journal of veterinary medical science / the Japanese Society of Veterinary Science, Vol. 73, Issue 6, pp. 821-6, (2011) (PubMed).

Yamada, Ryo, Tai, Tamaki, Inoue, Mishima, Tsubota, Saito: "Evaluation of therapeutic effects of astaxanthin on impairments in salivary secretion." in: Journal of clinical biochemistry and nutrition, Vol. 47, Issue 2, pp. 130-7, (2010) (PubMed).

Wakamatsu, Dogru, Ayako, Takano, Matsumoto, Ibrahim, Okada, Satake, Fukagawa, Shimazaki, Tsubota, Fujishima: "Evaluation of lipid oxidative stress status and inflammation in atopic ocular surface disease." in: Molecular vision, Vol. 16, pp. 2465-75, (2010) (PubMed).

Fukuchi, Miura, Nabeno, Kato, Osawa, Naito: "Immunohistochemical detection of oxidative stress biomarkers, dityrosine and N(epsilon)-(hexanoyl)lysine, and C-reactive protein in rabbit atherosclerotic lesions." in: Journal of atherosclerosis and thrombosis, Vol. 15, Issue 4, pp. 185-92, (2008) (PubMed).

Nagotani, Hayashi, Sato, Zhang, Deguchi, Nagano, Shoji, Abe: "Reduction of cerebral infarction in stroke-prone spontaneously hypertensive rats by statins associated with amelioration of oxidative stress." in: Stroke; a journal of cerebral circulation, Vol. 36, Issue 3, pp. 670-2, (2005) (PubMed).

Ueno, Horio, Uchida, Naito, Nomura, Kato, Tsuda, Toyokuni, Osawa: "Increase in oxidative stress in kidneys of diabetic Akita mice." in: Bioscience, biotechnology, and biochemistry, Vol. 66, Issue 4, pp. 869-72, (2002) (PubMed).

Target Details

Target: N epsilon Hexanoyl-Keyhole Limpet Hemocyanin (HEL-KLH)

Background: Oxidative damage of lipids caused by reactive oxygen species (ROS) play an important role in some diseases, lesion of cell functions and aging. Aldehydes such as malondi-aldehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE) have been reported as one of the advanced lipid peroxidation products. But recently in the earlier stage of lipid peroxidation, 13-hydroperoxyoctadecanoic acid (13-HPODE) is found to be covalently bound to proteins1). Hexanoyl-Lysine adduct (HEL) is a novel lipid hydroperoxide-modified lysine residues. HEL is formed by oxidative modification by oxidized omega-6 fatty acids such as linoleic acid or arachidonic acid. This monoclonal antibody (clone 5H4)is spesific for proteins and peptides containing HEL. Suitable for HEL detection by immunohistochemistry and western blotting.