Metabotropic Glutamate Receptor 5 antibody (AA 824-1203)

Catalog No. ABIN1027721

The Mouse Monoclonal anti-Metabotropic Glutamate Receptor 5 antibody has been validated for WB, IHC, IF, IP and ICC. It is suitable to detect Metabotropic Glutamate Receptor 5 in samples from Rat.

Quick Overview for Metabotropic Glutamate Receptor 5 antibody (AA 824-1203) (ABIN1027721)

Target: Metabotropic Glutamate Receptor 5 (GRM5) (Glutamate Receptor, Metabotropic 5 (GRM5))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Metabotropic Glutamate Receptor 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: S75-33

Product Details anti-Metabotropic Glutamate Receptor 5 Antibody

Binding Specificity: AA 824-1203

Specificity: Detects ~130 kDa (both mGluR1 and mGluR5).

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein amino acids 824-1203 (cytoplasmic C-terminus) of rat mGluR5b

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN1027721 was sufficient for detection of mGluR1/5 glutamate receptor in 20 μg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

Target Details for Metabotropic Glutamate Receptor 5

Target: Metabotropic Glutamate Receptor 5 (GRM5) (Glutamate Receptor, Metabotropic 5 (GRM5))

Alternative Name: mGluR5

Background: The AMPA receptor is a non-NMDA-type ionotropic transmembrane receptor for glutamate that mediates fast synaptic transmission in the CNS. AMPARs are composed of four types of subunits, designated as GluR1, GluR2, GluR3 and GluR4, which combine to form tetramers (1,2).

Gene ID: 24418

NCBI Accession: NP_058708

UniProt: P31424

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity