Rec.Pr.Thyrosin Phosphate (RPTP) antibody

Catalog No. ABIN1042487

Product Details

Target: Rec.Pr.Thyrosin Phosphate (RPTP)

Reactivity: Human, Pig, Rat

Host: Mouse

Clonality: Monoclonal

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Specificity: Monoclonal antibodies 3G4 and 3D7 have been directed against the extracellular domain of RPTPµ.

Characteristics: Cell adhesion

Purification: Purified

Clone: 3D7

Isotype: IgG2a

Application Details

Application Notes: 3D7 is useful for western blot, immunofluorescence, immunoprecipitation and flow cytometry. Optimal antibody dilution should be determined by titration.

Comment:

3D7 is a mouse monoclonal IgG2a antibody derived by fusion of SP2/0 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with ExG protein, secreted by NIH-3T3 transfected cells and purified with glutahione beads. The cells have been transfected using a fusion construct, termed ExG, encoding the entire ectodomain of the human RPTPµ molecule (aa 1-742), fused COOH terminally to GST.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.09% sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References

Zondag, Reynolds, Moolenaar: "Receptor protein-tyrosine phosphatase RPTPmu binds to and dephosphorylates the catenin p120(ctn)." in: The Journal of biological chemistry, Vol. 275, Issue 15, pp. 11264-9, (2000) (PubMed).

Target Details

Target: Rec.Pr.Thyrosin Phosphate (RPTP)

Background: Receptor-like protein tyrosine phosphatases (RPTPs) represent a new family of transmembrane proteins that are thought to transduce external signals by dephosphorylating phosphotyrosine residues on intracellular substrates. RPTPµ is a prototypic receptor-like protein-tyrosine phosphatase (RPTP) that mediates homotypic cell-cell interactions. Intracellularly, RPTPµ consists of a relatively large juxtamembrane region (about 150 amino acids) and two conserved phosphatase domains. As in most RPTPs, the first (membrane-proximal) PTP domain of RPTPµ is catalytically active, whereas its second (C-terminal) domain is inactive. RPTPµ is involved in cell-cell adhesion through homophilic interactions and may also play a key role in signal transduction and growth control.