Serotonin Receptor 4 antibody (2nd Extracellular Loop)

Catalog No. ABIN7887155

The Rabbit Polyclonal anti-Serotonin Receptor 4 antibody is suitable to detect Serotonin Receptor 4 in samples from Rat. It has been validated for WB, FACS, LCI and IC.

Quick Overview for Serotonin Receptor 4 antibody (2nd Extracellular Loop) (ABIN7887155)

Target: Serotonin Receptor 4 (HTR4)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Serotonin Receptor 4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI), Immunochromatography (IC)

Grade: KO Validated

Product Details anti-Serotonin Receptor 4 Antibody

Binding Specificity: 2nd Extracellular Loop, AA 168-180

Purpose: A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 4

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - 12,13 amino acid residues identical, human - 11

Characteristics: Anti-5HT4 Receptor (HTR4) (extracellular) Antibody (ABIN7043624, ABIN7045290 and ABIN7045291) is a highly specific antibody directed against an epitope of the rat Serotonin receptor 4. The antibody can be used in western blot, immunocytochemistry, and live cell imaging applications.  It has been designed to recognize 5HT4 receptor from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)DVIEKRKFNHNSN, corresponding to amino acid residues 168-180 of rat 5-Hydroxytryptamine receptor 4

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 mg per reaction

ICC: 1:100

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Serotonin Receptor 4

Target: Serotonin Receptor 4 (HTR4)

Alternative Name: HTR4

Background:

Synonyms: 5-Hydroxytryptamine receptor 4, Serotonin receptor 4

Description: 5-hydroxytryptamine receptor 4 (5-HT4 receptors) are a subclass among the large family of receptors mediating serotonin actions at central and peripheral targets. 5-HT4 receptor activation has been implicated in learning and memory, feeding control and stress response. 5-HT4 receptors are encoded by a complex gene which in humans, generates ten different splice variants1, 2.The 5-HT4 receptor, a member of the seven transmembrane spanning G-protein-coupled receptors (GPCRs), couples via Gs to increase cAMP production. GPCRs share a similar topology, with seven transmembrane helices (TMHs) connected by three extracellular loops (ECLs), and three intracellular loops (ICLs), the N-terminus is on the extracellular side of the membrane, and the C-terminus is on the cytoplasmic side3.Within the central nervous system, these receptors are expressed in limbic structures including: hypothalamus, nucleus accumbens, prefrontal cortex, amygdala and hippocampus, where they contribute to emotional and cognitive functions4.Low levels of 5-HT4 receptors are associated with numerous neuropsychiatric disorders including Alzheimer's disease, Huntington's disease, feeding disorders and stress-related disorders such as anxiety and depression5.

Gene ID: 25324

NCBI Accession: NM_000870

UniProt: Q62758

Pathways: JAK-STAT Signaling, cAMP Metabolic Process, S100 Proteins