EPH Receptor B2 antibody (Internal Region)

Catalog No. ABIN1048573

Product Details anti-EPH Receptor B2 Antibody

Target: EPH Receptor B2 (EPHB2)

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Monkey, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPH Receptor B2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human EPHB2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Panda, Rabbit, Opossum, Zebrafish (100%) Hamster, Elephant, Bovine, Pig, Turkey, Chicken, Pufferfish (93%) Xenopus, Seabass (87%) Stickleback (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 15 amino acid peptide from internal region of human EPHB2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Panda, Rabbit, Opossum, Zebrafish (100%), Hamster, Elephant, Bovine, Pig, Turkey, Chicken, Pufferfish (93%), Xenopus, Seabass (87%), Stickleback (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (7 μg/mL)

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for EPH Receptor B2

Target: EPH Receptor B2 (EPHB2)

Alternative Name: EPHB2 / EPH Receptor B2 (EPHB2 Products)

Synonyms: CAPB antibody, DRT antibody, EK5 antibody, EPHT3 antibody, ERK antibody, Hek5 antibody, PCBC antibody, Tyro5 antibody, ERK2 antibody, ERT1 antibody, MAPK2 antibody, P42MAPK antibody, PRKM1 antibody, PRKM2 antibody, p38 antibody, p40 antibody, p41 antibody, p41mapk antibody, RGD1564232 antibody, Cek5 antibody, Drt antibody, ETECK antibody, Erk antibody, Nuk antibody, Prkm5 antibody, Qek5 antibody, Sek3 antibody, cek5 antibody, ephb2 antibody, xEphB2 antibody, si:ch73-160e9.1 antibody, tpx2 antibody, wu:fb09e10 antibody, wu:fc64a03 antibody, wu:fi18g12 antibody, zgc:112296 antibody, EPH receptor B2 antibody, mitogen-activated protein kinase 1 antibody, Eph receptor B2 antibody, EPH receptor B2 L homeolog antibody, eph receptor B2a antibody, eph receptor B2b antibody, EPHB2 antibody, MAPK1 antibody, Ephb2 antibody, ephb2.L antibody, ephb2a antibody, ephb2b antibody

Background: Name/Gene ID: EPHB2
Subfamily: Ephrin Receptor
Family: Protein Kinase

Synonyms: EPHB2, CAPB, Cek5, EK5, ETECK, EPH tyrosine kinase 3, DRT, ELK-related tyrosine kinase, Hek5, EPH-like kinase 5, EPHT3, Nuk, PCBC, Tyrosine-protein kinase TYRO5, Protein-tyrosine kinase HEK5, Tyro5, EPH receptor B2, Ephrin type-B receptor 2, EPTH3

Gene ID: 2048

Pathways: RTK Signaling, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins