PCK1 antibody

Catalog No. ABIN105565

Supplier Product No.: 200-4163-0100

This anti-PCK1 antibody is a Rabbit Polyclonal antibody detecting PCK1 in WB, ELISA and IHC. Suitable for Zea mays.

Quick Overview for PCK1 antibody (ABIN105565)

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Reactivity: Zea mays

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-PCK1 Antibody

Supplier Product No.: 200-4163-0100

Supplier: Rockland

Purpose: Phospho Enol Pyruvate Carboxylase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Phospho-enol-Pyruvate Carboxylase [Maize].

Characteristics: Synonyms: rabbit anti-Phospho Enol Pyruvate Carboxylase Antibody, rabbit anti-PEPC 1 antibody, PEPCase 1 antibody, Phosphoenolpyruvate carboxylase 1 antibody

Purification: Anti-Phospho Enol Pyruvate Carboxylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Anti-Phospho Enol Pyruvate Carboxylase Antibody was produced by repeated immunizations with maize leaves Phospho-enol-pyruvate Carboxylase.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-Phospho Enol Pyruvate Carboxylase antibody has been tested by western blotting and ELISA is suitable for IHC. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:1,000 - 1:4,000

ELISA Dilution: 1:10,000 - 1:40,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for PCK1

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Alternative Name: Phospho Enol Pyruvate Carboxylase

Background: Background: Anti-Phospho Enol Pyruvate Carboxylase antibody detects PEP. Phosphoenolpyruvate carboxylase is an enzyme in the family of carboxy-lyases that catalyzes the addition of bicarbonate to phosphoenolpyruvate (PEP) to form the four-carbon compound oxaloacetate. This reaction is used for carbon fixation in so-called "CAM" and "C4" plants where it plays a key role in photosynthesis. The enzyme is also found in some bacteria, but not in animals or fungi. Anti-Phospho Enol Pyruvate Carboxylase Antibody is ideal for investigators involved in Cell Signaling, biochemistry and Signal Transduction research.

Gene ID: 542372

UniProt: B8XPZ2

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis