PPARG antibody (AA 250-300)

Catalog No. ABIN105800

Supplier Product No.: 600-401-419

The Rabbit Polyclonal anti-PPARG antibody has been validated for WB and ELISA. It is suitable to detect PPARG in samples from Human, Mouse, Rat, Dog, Rabbit, Guinea Pig, Pig, Hamster, Duck, Mink, Squirrel, Macaque and Orang-Utan. There are 3+ publications available.

Quick Overview for PPARG antibody (AA 250-300) (ABIN105800)

Target: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Guinea Pig, Pig, Hamster, Duck, Mink, Squirrel, Macaque, Orang-Utan

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPARG antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PPARG Antibody

Binding Specificity: AA 250-300

Supplier Product No.: 600-401-419

Supplier: Rockland

Purpose: PPAR gamma 1 + 2 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human PPAR gamma protein.

Characteristics: Synonyms: rabbit anti-PPAR gamma 1 and 2 antibody, Peroxisome proliferator-activated receptor gamma, Nuclear receptor subfamily 1 group C member 3, NR1C3, PPARG, PPAR gamma, PPARG1 antibody, PPARG2 antibody

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 250-300 of human PPAR gamma isoform 1.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity-purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User optimized

ELISA Dilution: 1:8,000 - 1:32,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-PPARG antibody (ABIN105800)

Fei, Tamski, Cook, Santanam: "MicroRNA regulation of adipose derived stem cells in aging rats." in: PLoS ONE, Vol. 8, Issue 3, pp. e59238, (2013) (PubMed).

Moreau, Téruel, Beylot, Albalea, Tamasi, Umbdenstock, Parmentier, Sa-Cunha, Suc, Fabre, Navarro, Ramos, Meyer, Maurel, Vilarem, Pascussi: "A novel pregnane X receptor and S14-mediated lipogenic pathway in human hepatocyte." in: Hepatology (Baltimore, Md.), Vol. 49, Issue 6, pp. 2068-79, (2009) (PubMed).

Bruedigam, Koedam, Chiba, Eijken, van Leeuwen: "Evidence for multiple peroxisome proliferator-activated receptor gamma transcripts in bone: fine-tuning by hormonal regulation and mRNA stability." in: FEBS letters, Vol. 582, Issue 11, pp. 1618-24, (2008) (PubMed).

Target Details for PPARG

Target: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Alternative Name: PPARG

Background: Background: Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing thiazolidinediones.  Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes. There are 3 known subtypes of PPARs: PPAR-alpha, PPAR-delta and PPAR-gamma.  Mostly target genes are involved in the catabolism of fatty acids. Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and anti-diabetic thiazolidinediones and affects the expression of genes involved in the storage of the fatty acids. PPAR-gamma may also be involved in adipocyte differentiation. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements.

Gene ID: 5468, 20336229

UniProt: P37231

Pathways: MAPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation