Pyruvate Kinase antibody

Catalog No. ABIN106387

Product Details anti-Pyruvate Kinase Antibody

Target: Pyruvate Kinase (PK)

Reactivity: Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This Pyruvate Kinase antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Conjugation (Con), Dot Blot (DB)

Characteristics: Concentration Definition: by Refractometry

Purification: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Pyruvate Kinase [Rabbit Muscle].

Immunogen: Pyruvate Kinase [Rabbit Muscle]
Immunogentype:Native

Application Details

Application Notes: Anti-PYRUVATE KINASE Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. Specific conditions should be optimized by user.

Comment:

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent)

Concentration: 40 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-Pyruvate Kinase antibody (ABIN106387)

Shimada, Shinagawa, Ishii: "Modulation of M2-type pyruvate kinase activity by the cytoplasmic PML tumor suppressor protein." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 13, Issue 3, pp. 245-54, (2008) (PubMed).

Target Details for Pyruvate Kinase

Target: Pyruvate Kinase (PK)

Alternative Name: Pyruvate Kinase (PK Products)

Synonyms: CG7070 antibody, Dmel\\CG7070 antibody, PYK antibody, Pyk antibody, PYK3b antibody, PYK3c antibody, Pyruvate kinase antibody, PK antibody, pyruvate kinase antibody, pyruvate kinase (PK) antibody, Pyruvate kinase (PK) antibody, pyruvate kinase PKM antibody, testis expressed 45 antibody, PyK antibody, pykA antibody, PYK3a|PYK3b|PYK3c antibody, pyk antibody, pyk1 antibody, pk antibody, LOC100534543 antibody, TEX45 antibody

Background: Pyruvate kinase (EC: 2.7.1.40) is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. This process also requires a magnesium ion. The enzyme is a 'transferase' under the international classification of enzymes. This step is the final one in the glycolytic pathway, which produces pyruvate molecules, the final product of aerobic glycolysis. However, in anaerobic glycolysis, lactate dehydrogenase will utilize the NADH produced by glyceraldehyde phosphate dehydrogenase to reduce pyruvate to lactate. In humans, there are two pyruvate kinase isozymes: type M (muscle, SwissProt P14618) and type L,R (liver and erythrocyte, SwissProt P30613). The isozymes differ in primary structure and regulation. This reaction has a large positive free energy change, one of three in glycolysis. All three such steps regulate the overall activity of the pathway, and are, in general, irreversible under wild-type conditions. Anti-Pyruvate kinase antibody is idea for investigators involved in cytokines and growth factor research.
Synonyms: Pyruvate kinase isozymes M1/M2 antibody, Pyruvate kinase muscle isozyme antibody, Pyruvate kinase 2/3 antibody, Cytosolic thyroid hormone-binding protein antibody

Gene ID: 100008676

NCBI Accession: NP_001182574

UniProt: P11974