Superoxide Dismutase antibody

Catalog No. ABIN107158

Product Details

Target: Superoxide Dismutase

Reactivity: Cow, Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): Cross reactivity against Superoxide Dismutase from other tissues and species may occur but have not been specifically determined.

Purity: Anti-SUPEROXIDE DISMUTASE (SOD) is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Superoxide Dismutase [Bovine erythrocytes].

Endotoxin Level: Low Endotoxin : No

Immunogen: Superoxide Dismutase [Bovine erythrocytes]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: SUPEROXIDE DISMUTASE (SOD) Antibody has been assayed against 1.0 µg of Superoxide Dismutase [Bovine erythrocytes] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:10.000 to 1:50.000 is suggested for this product.
ELISA Dilution: 1:10.000 - 1:50.000
Western Blot Dilution: 1:500 - 1:2.000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details

Target: Superoxide Dismutase

Synonyms: GTP-binding nuclear protein Ran antibody, superoxide dismutase 1 antibody, LOC786258 antibody, SOD1 antibody

Background: Synonyms: ALS 1 antibody, ALS antibody, ALS1 antibody, Amyotrophic lateral sclerosis 1 adult antibody, Amyotrophic lateral sclerosis 1 antibody, Cu/Zn SOD antibody, Cu/Zn superoxide dismutase antibody, Homodimer antibody, SOD

Gene ID: 281495

UniProt: P00442