WIPI2 antibody (N-Term)

Catalog No. ABIN1077403

Product Details anti-WIPI2 Antibody

Target: WIPI2 (WD Repeat Domain, Phosphoinositide Interacting 2 (WIPI2))

Binding Specificity: N-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WIPI2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: WIPI2 antibody is human and rat reactive. Multiple isoforms of WIPI2 are known to exist. WIPI2 antibody is predicted to not cross-react with WIPI1.

Purification: Affinity chromatography purified via peptide column

Immunogen: Rabbit polyclonal WIPI2 antibody was raised against a 17 amino acid peptide near the amino terminus of human WIPI2.

Isotype: IgG

Application Details

Application Notes: WIPI2 antibody can be used for detection of WIPI2 by Western blot at 1 - 2 μg/mL.

Comment:

(NT) WD repeat domain phosphoinositide interacting 2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Storage: 4 °C/-20 °C

Storage Comment: WIPI2 antibody can be stored at 4 °C for three months. At -20 °C stable for up to one year.

Expiry Date: 12 months

Target Details for WIPI2

Target: WIPI2 (WD Repeat Domain, Phosphoinositide Interacting 2 (WIPI2))

Alternative Name: WIPI2 (WIPI2 Products)

Synonyms: ATG18B antibody, Atg21 antibody, WIPI-2 antibody, 1110018O08Rik antibody, 2510001I10Rik antibody, WD repeat domain, phosphoinositide interacting 2 L homeolog antibody, WD repeat domain, phosphoinositide interacting 2 antibody, wipi2.L antibody, WIPI2 antibody, Wipi2 antibody

Background: WD repeat proteins play a role in many essential biologic functions, regulating the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. WIPI2, also known as ATG18B or ATG21, is a human homolog to yeast ATG18 and contains three WD repeats and has a 7-bladed propeller structure with a conserved motif that facilitates its interaction with other proteins. It is recruited to early autophagosomal structures along with Atg16L and ULK1 and is required for the formation of LC3-positive autophagosomes. Along with the highly related WIPI1, WIPI2 is found at the plasma membrane in addition to autophagosomal membranes.
Synonyms: WD repeat domain phosphoinositide interacting 2, WIPI-2, WD40 repeat protein interacting with phosphoinositides 2, WIPI49-like protein 2, ATG18B, ATG21, CGI-50

Molecular Weight: 50 kDa

Gene ID: 26100

NCBI Accession: NP_056425