PLK1 antibody (AA 331-508)

Catalog No. ABIN1098105

This anti-PLK1 antibody is a Mouse Monoclonal antibody detecting PLK1 in WB, ELISA, IHC and FACS. Suitable for Human and Mouse. This Primary Antibody has been cited in 3+ publications.

Quick Overview for PLK1 antibody (AA 331-508) (ABIN1098105)

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This PLK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: 3C11

Product Details anti-PLK1 Antibody

Binding Specificity: AA 331-508

Purpose: PLK1 Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human PLK1 (AA: 331-508) expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-PLK1 antibody (ABIN1098105)

Lin, Sun, Wang: "Suppression of Polo like kinase 1 (PLK1) by p21(Waf1) mediates the p53-dependent prevention of caspase-independent mitotic death." in: Cellular signalling, Vol. 23, Issue 11, pp. 1816-23, (2011) (PubMed).

Yoshioka, Kimura, Saio, Era, Okano: "Plk1 is negatively regulated by RNF8." in: Biochemical and biophysical research communications, Vol. 410, Issue 1, pp. 57-61, (2011) (PubMed).

Susilowati, Okamura, Hirota, Shono, Yoshida, Murakami, Tabata, Nagamune, Haneji, Miyake: "Intermedilysin induces EGR-1 expression through calcineurin/NFAT pathway in human cholangiocellular carcinoma cells." in: Biochemical and biophysical research communications, Vol. 404, Issue 1, pp. 57-61, (2011) (PubMed).

Target Details for PLK1

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Alternative Name: PLK1

Background: Polo-like kinase 1 (Plk1) plays key roles in many aspects of mitosis. Suppression of Plk1 by p21(Waf1) is responsible for p53-dependent protection against adriamycin-induced caspase-independent mitotic death.

Molecular Weight: 68 kDa

Gene ID: 5347

UniProt: P53350

Pathways: Cell Division Cycle, M Phase