PYCARD antibody

Catalog No. ABIN1105662

Product Details anti-PYCARD Antibody

Target: PYCARD (PYD and CARD Domain Containing (PYCARD))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PYCARD antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Specificity: This antibody reacts with human ASC of U937, and HL-60, but not with Jurkat, HeLa, NB4, or HPB-ALL. It does not react with mouse NIH/3T3, L5178Y, or WR19L. It does not react with rat PC12.

Purification: Protein A agarose

Immunogen: Triton X-100 insoluble fraction of retinoic acid-treated HL-60 cells

Clone: 23-04-14

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 50 % glycerol, pH 7.2. No preservative is contained.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store (in aliquots) at -20 °C.

Target Details for PYCARD

Target: PYCARD (PYD and CARD Domain Containing (PYCARD))

Alternative Name: CARD5 / PYCARD / ASC (PYCARD Products)

Synonyms: ASC antibody, CARD5 antibody, TMS antibody, TMS-1 antibody, TMS1 antibody, 9130417A21Rik antibody, Asc antibody, TNS1 antibody, masc antibody, asc1 antibody, zAsc antibody, zgc:109874 antibody, PYD and CARD domain containing antibody, PYCARD antibody, Pycard antibody, pycard antibody

Background: ASC (apoptosis-associated speck-like protein containing a CARD (caspase recruitment domain)) is a 22 kDa soluble protein, located in the cytosol of HL-60 cells. In apoptotic HL-60 cells, it is able to visualized as a speck, forming an insoluble aggregate. The C-terminal domain of this protein contains a CARD, suggesting that ASC may have proapoptotic activity in HL-60 cells. Recent data have indicated that ASC plays a role of adaptor protein linking various PAAD (Pyrin, AIM, ASC, and death domain-like) family proteins to pathways involved in NF-κB and procaspase-1 activation.Synonyms: Caspase recruitment domain-containing protein 5, PYD and CARD domain-containing protein, TMS1, Target of methylation-induced silencing 1, poptosis-associated speck-like protein containing a CARD

Molecular Weight: 22 kDa

Gene ID: 29108

NCBI Accession: NP_037390

UniProt: Q9ULZ3

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation, Inflammasome