CD11b antibody (FITC)

Catalog No. ABIN7101947

Product Details anti-CD11b Antibody (FITC)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD11b antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: This antibody clone VIM12 (clone renamed, formerly Bear-1) reacts with the CD11b alpha chain of the CD11b/CD18 (p165, 95) integrin heterodimer complex present on Human NK cells, monocytes, and granulocytes. The recognized structure is the alpha chain of a heterodimer glycoprotein of Mw 165 kDa (alpha chain) and 95 kDa (beta chain).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Characteristics: Absorption / Emission: 488 nm / 525 nm

Clone: VIM12

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.09 % Sodium Azide Highly purified grade of BSA has been added as a stabilizing protein to bring the final protein concentration to 4-5 mg/mL.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE! This product is photosensitive and should protected from light.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Alternative Name: CD11b (ITGAM Products)

Background: CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8 % spleen cells, 44 % bone marrow cells and less than 1 % of thymocytes and is commonly used as a microglial marker in nervous tissue.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor

Gene ID: 3684

NCBI Accession: NP_000623

UniProt: P11215

Pathways: Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation