CD130/gp130 antibody

Catalog No. ABIN1105841

The Rat Monoclonal anti-CD130/gp130 antibody is suitable to detect CD130/gp130 in samples from Mouse. It has been validated for FACS.

Quick Overview for CD130/gp130 antibody (ABIN1105841)

Target: CD130/gp130 (IL6ST) (Interleukin 6 Signal Transducer (Gp130, Oncostatin M Receptor) (IL6ST))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD130/gp130 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: RMb1

Product Details anti-CD130/gp130 Antibody

Cross-Reactivity (Details): Species reactivity (tested):Mouse

Purification: Ammonium sulfate precipitation and affinity chromatography on protein G

Immunogen: Recombinant mouse gp130 expressed in 293T cells

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Reconstitute with 100 μL of distilled water.

Buffer: PBS with 10 mg/mL sucrose, 1 μg/mL sodium azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store (in aliquots) at -20 °C up to three months.

Target Details for CD130/gp130

Target: CD130/gp130 (IL6ST) (Interleukin 6 Signal Transducer (Gp130, Oncostatin M Receptor) (IL6ST))

Alternative Name: CD130 / IL6ST

Background: The interleukin-6 (IL-6) cytokine family, including IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF), cardiotrophin-1 (CT-1), and B-cell stimulatory factor 3, are also known as gp130 cytokines because they all share gp130 as a common transducer protein within their functional receptor complexes. In the case of IL-6, binding of the ligand to its specific a-receptor (IL-6Ra/CD126) induces dimerization of gp130, activation of the gp130-associated Janus kinases Jak1, Jak2 and Tyk2 and phosphorylation of gp130 at cytoplasmic tyrosine residues. These phosphotyrosines serve as docking sites for SH2-domain-containing proteins such as STAT1, STAT3, the protein tyrosine phosphatase SHP2 and the suppressor of cytokine signaling 3 (SOCS3). The subsequently tyrosine phosphorylated STAT homo- or heterodimers translocate to the nucleus where they bind to enhancer sequences of IL-6-inducible genes. Both IL-6Ra and gp130 have soluble forms. Although soluble gp130 inhibits the effect of IL-6, the soluble IL-6Ra promotes the effect of IL-6.Synonyms: IL-6R-beta, IL6 receptor beta, Interleukin-6 receptor subunit beta

Gene ID: 10090

UniProt: Q00560

Pathways: JAK-STAT Signaling, Cellular Glucan Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Cancer Immune Checkpoints