CD5 antibody (PE)

Catalog No. ABIN1106441

Product Details anti-CD5 Antibody (PE)

Target: CD5

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD5 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Specificity: MCD5 recognizes a 67 kDa antigen on human T cells. Other species not tested. Testing by flow cytometry using a 'lyse-wash' method on whole blood from healthy donors showed the following values expressed in terms of % of the total lymphocyte count: Product code: AM39009RP-N (anti-CD5 PE) Mean % positive: 73,32 S.D.: 5,58 % CV: 7,61

Purification: Affinity chromatography

Clone: MCD5

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: 0.01 M sodium phosphate, 0.15 M NaCl, pH 7.3, 0.2 % BSA, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. This product is photosensitive and should be protected from light.

Target Details for CD5

Target: CD5

Alternative Name: CD5 (CD5 Products)

Synonyms: CD5 antibody, Ly-1 antibody, Ly-12 antibody, Ly-A antibody, Lyt-1 antibody, LEU1 antibody, T1 antibody, CD5 molecule antibody, CD5 antigen antibody, CD5 molecule like antibody, Cd5 molecule antibody, CD5 antibody, Cd5 antibody, CD5L antibody

Background: CD5 appears to be a relatively late marker during B cell differentiation. CD5 expression is thought to be absent on surface Ig negative B-lineage cells but appears on IgM+ cells in both fetal liver and bone marrow. It co-precipitates with the T cell receptor and, in particular with Lck. A role for CD5 in signal transduction is postulated based on stimulatory effects of CD5 monoclonal antibodies. CD5 antigen is phosphorylated on tyrosine residues on T cell activation. There is evidence that CD5 plays a role in thymocyte selection, as well as a role in cell-cell recognition. Recently CD5 on B cells has been shown to be an endogenous ligand selective for B-cell surface IgFR (framework region) sequences. Interaction of surface Ig with CD5, other endogenous antigens or (in mucosal sites) exogenous superantigens can provide B cells with continual stimulation and might prevent their elimination from the immune system. In addition, B cell superantigens, e.g. Staphylococcus aureus Cowan strain 1, may contribute to the pathogenesis of autoimmune diseases and malignancies.Synonyms: CD5, LEU1, Lymphocyte antigen T1/Leu-1, T-cell surface glycoprotein CD5

Gene ID: 921

NCBI Accession: NP_055022

UniProt: P06127