Collagen IV antibody

Catalog No. ABIN1106751

This anti-Collagen IV antibody is a Mouse Monoclonal antibody detecting Collagen IV in IHC (fro) and EIA. Suitable for Human.

Quick Overview for Collagen IV antibody (ABIN1106751)

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Collagen IV antibody is un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: MC4-HA

Product Details anti-Collagen IV Antibody

Specificity: This monoclonal antibody reacts with native human collagen type IV, and with heat- denatured collagen IV form in ELISA and immunoblots. There is no cross-reactivity with human collagen I,II,III and V. It stains exclusively basement membranes on cryostat sections of human tissues such as kidney, liver and heart. The extent of expression of the basement membrane component type IV collagen may be correlated with the degree of differentiation of basement membrane producing neoplasms. Neoplastic cells of invasive carcinomas often lack a continuous basement membrane. In diagnostic histopathology antibodies to type IV collagen may help to distinguish between non-invasive (benign or in situ) and invasive lesions. Furthermore the antibody is important in the differential diagnosis of bullous lesions in dermatopathology. In in vitro studies with endothelial cells and smooth muscle cells the antibody is helpful in detection of production collagen type IV. This antibody reacts also with dog collagen IV on cryostate and paraffin sections.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: PEG6000 precipitation + Sepharose 6B gel filtration

Isotype: IgM

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Reconstitute with 1 mL sterile water and add preservative if preferred.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Alternative Name: Collagen Type IV

Background: Synonyms: COL4A1

Gene ID: 1282

NCBI Accession: NP_001836

UniProt: P02462