IL-2 antibody (PE)

Catalog No. ABIN1107823

This Mouse Monoclonal antibody specifically detects IL-2 in FACS. It exhibits reactivity toward Human.

Quick Overview for IL-2 antibody (PE) (ABIN1107823)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-2 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: N7-48A

Product Details anti-IL-2 Antibody (PE)

Specificity: The antibody detects human Interleukin 2, which is a 15 kDa molecular weight glycoprotein. Other species not tested.

Purification: Affinity chromatography

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: 0.01 M sodium phosphate, 0.15 M NaCl, pH 7.3, 0.2 % BSA, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. Fluorochrome labelled product is photosensitive and should be protected from light.

Target Details for IL-2

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: Interleukin-2 / IL2

Background: The immune system reacts to a pathogen by activation of balanced network of the humoral and cellular immune responses. Subsequently the activated condition of the immune system will, after the elimination of the pathogen, be down-regulated to a balanced situation again. Control of the immune response requires efficient communication between the different cells involved in this response. This interaction is provided by cell/cell contact and by a complex array of mediators. Among these mediators cytokines, soluble factors produced by these cells, play an important role. Cytokines can act on other cells locally or distantly, but can be even auto regulating. Cytokines can behave stimulatory or inhibitory, or can even perform both activities, depending on the (pre)activation stage of the target cell. (3, 4) Interleukine 2 (IL-2) is a cytokine expressed by human lymphocytes that have been stimulated by antigens or mitogens. Without stimulation, IL-2 is produced in low amounts by TH1 cells in vivo. Originally, IL-2 was referred to as T cell growth factor. T cell hybridomas or a leukemia cell line such as Jurkat, produce increased amounts of IL-2 in vitro. Stimulation with IL-2 leads to rapid clonal expansion of T cells and IL-2 acts on a variety of cells in vivo, such as B cells, NK cells, monocytes and macrophages. Furthermore, IL-2 can induce activation of LAK cells.Synonyms: IL-2, T-cell growth factor, TCGF

Gene ID: 3558

NCBI Accession: NP_000577

UniProt: P60568

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation