Paxillin antibody (C-Term)

Catalog No. ABIN1108595

The Mouse Monoclonal anti-Paxillin antibody is suitable to detect Paxillin in samples from Human, Mouse, Rat and Chicken. It has been validated for WB and IF.

Quick Overview for Paxillin antibody (C-Term) (ABIN1108595)

Target: Paxillin (PXN)

Reactivity: Human, Mouse, Rat, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This Paxillin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: PAX-14

Product Details anti-Paxillin Antibody

Binding Specificity: AA 305-559, C-Term

Specificity: This antibody reacts to Paxillin.

Purification: Affinity chromatography

Immunogen: C-terminal part of recombinant chicken paxillin (amino acids 305-559)

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.2 % sodium acetate or neutral PBS

Concentration: 0,1 mg/mL

Buffer: 1.2 % sodium acetate, with 2 mg BSA and 0.01 mg sodium azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Prior to reconstitution store at -20 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Paxillin

Target: Paxillin (PXN)

Alternative Name: Paxillin / PXN

Background: The paxillin gene can be alternatively spliced to include 1 of 2 alternative exons, generating beta and gamma isoforms. Paxillin is a 68- kDa focal adhesion protein that is phosphorylated on tyrosine residues in fibroblasts in response to transformation by v-src, treatment with platelet-derived growth factor, or cross-linking of integrins. The 68-kD protein (paxillin) is a cytoskeletal component that localizes to the focal adhesions at the ends of actin stress fibers in chicken embryo fibroblasts. It is also present in the focal adhesions of Madin-Darby bovine kidney (MDBK) epithelial cells but is absent, like talin, from the cell-cell adherens junctions of these cells.Synonyms: FLJ16691

Gene ID: 5829

NCBI Accession: NP_001074324

UniProt: P49023

Pathways: MAPK Signaling, EGFR Signaling Pathway, Response to Growth Hormone Stimulus, Cell-Cell Junction Organization, Maintenance of Protein Location, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling