Fibrinogen antibody

Catalog No. ABIN110984

The Mouse Monoclonal anti-Fibrinogen antibody has been validated for WB and EIA. It is suitable to detect Fibrinogen in samples from Human.

Quick Overview for Fibrinogen antibody (ABIN110984)

Target: Fibrinogen

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Fibrinogen antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: FgA

Product Details anti-Fibrinogen Antibody

Purification: Gel filtration and anion exchange chromatography

Immunogen: Purified human Fibrinogen

Isotype: IgG1

Application Details

Application Notes: ELISA, RIA, westernblot (non-reducing conditions, in this application binds alpha-chain offibrinogen).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL sterile distilled water

Buffer: 10 mM Sodium Phosphate, 0.15 M NaCl, pH 7.4, 0.01 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2-8 °C for one month or (in small aliquots) at -20 °C for longer.

Target Details for Fibrinogen

Target: Fibrinogen

Background: Fibrinogen is the main protein of blood coagulation system. It is a large protein and it consists of two identical subunits that contain three polypeptide chains: alpha, beta and gamma. All chains are connected with each other by a number of disulfide bonds. Fibrinopeptides A (1 to 16 amino acids) and B (1 to 17 amino acids) are released by thrombin from the N terminal parts of alpha and beta chains, respectively. In this way fibrinogen is converted into fibrin, which by means of polymerization forms a fibrin clot. Fibrinogen clotting underlies pathogenesis of MI, thromboembolism and thromboses of arteries and veins, since fibrin is the main substrate for thrombus formation. Fibrinogen activation is also involved in pathogenesis of inflammation, tumor growth and many other diseases. The normal fibrinogen concentration in plasma is about 3 mg/mL. The elevated level of fibrinogen in patient's blood is regarded as an independent risk factor for cardiovascular diseases. An increase in blood fibrinogen concentration was shown to be a strong predictor of coronary heart disease. All these facts make fibrinogen an important parameter in the diagnosis of cardiovascular diseases.Synonyms: FGA, FGB, FGG