IGF1R antibody (Insulin-Like Growth Factor 1 Receptor) (Chain alpha)

Details for Product anti-IGF1R Antibody No. ABIN111141, Supplier: Log in to see
Antigen
  • CD221
  • IGFIR
  • IGFR
  • JTK13
  • IGFIRC
  • IGFR1
  • IGF-1R
  • IGF1R
  • DKFZp469I0618
  • igf-1r
  • xIGF-1R
  • xIGFR
  • xigf1r
  • A330103N21Rik
  • D930020L01
  • hyft
  • igf-ir
  • IGF-IRa
  • sb:eu1090
  • wu:fi20b10
  • insulin like growth factor 1 receptor
  • insulin-like growth factor 1 receptor
  • insulin-like growth factor I receptor
  • insulin like growth factor 1 receptor S homeolog
  • insulin-like growth factor 1a receptor
  • IGF1R
  • Igf1r
  • igf1r
  • LOC780503
  • LOC100136427
  • igf1r.S
  • igf1ra
Alternatives
anti-Human IGF1R antibody for Flow Cytometry
Epitope
Chain alpha
40
39
35
29
23
21
20
17
16
15
15
15
15
15
9
8
8
8
7
6
5
5
5
4
4
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
485
265
246
14
13
13
12
10
3
3
3
2
2
2
1
Host
Mouse
379
107
2
2
Clonality (Clone)
Monoclonal ()
Conjugate
This IGF1R antibody is un-conjugated
19
18
17
16
9
9
8
8
8
8
8
8
8
8
8
7
6
2
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
319
152
122
104
84
77
46
43
14
7
6
6
5
2
2
2
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Clone 1H7
Isotype IgG1
Specificity Clone 1H7 recognizes an epitope in the alpha subunits of CD221, demonstrated by Western blotting (Ref.1). Clone 1H7 is reported to partically block binding of IGF-I and IGF-II to CD221 (Ref.1)
Purification Affinity Chromatography on Protein G
Endotoxin Level Low Endotoxin
Plasmids, Primers & others Plasmids, Primers & others IGF1R products on genomics-online (e.g. as negative or positive controls)
Alternative Name CD221 / IGF1R (IGF1R Antibody Abstract)
Background CD221, a 155 kD tyrosine kinase, is composed of two extracellular alpha-subunits and two transmembrane beta-subunits. CD221 is expressed in a range of tissues, including kidney, liver, placenta, mammary gland, brain, ovary and skin. The ligands for CD221 include IGF-I and IGF-II, which bind to CD221 and activate tyrosine kinase activity, resulting in phosphorylation of several intracellular signalling proteins.Synonyms: IGF-I receptor, IGF1 Receptor, Insulin-like growth factor 1 receptor
Gene ID 3480
NCBI Accession NP_000866
UniProt P08069
Pathways RTK Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagy
Application Notes Functional Assays. Western blotting. Immunohistochemistry on Paraffin Sections: 1/10-1/40. This product does not requireprotein digestion pre-treatment of paraffin sections. Use pancreas or placenta as histologypositive control tissue. Flow Cytometry Use 10 μL of 1/10 diluted antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS without preservatives or stabilizers
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
Supplier Images
Flow Cytometry (FACS) image for anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (Chain alpha) antibody (ABIN111141) Staining of human peripheral blood granulocytes with mouse anti human CD221-low endot...
Product cited in: Hantel, Lewrick, Reincke, Süss, Beuschlein: "Liposomal doxorubicin-based treatment in a preclinical model of adrenocortical carcinoma." in: The Journal of endocrinology, Vol. 213, Issue 2, pp. 155-61, 2012 (PubMed).

Hantel, Lewrick, Schneider, Zwermann, Perren, Reincke, Süss, Beuschlein: "Anti insulin-like growth factor I receptor immunoliposomes: a single formulation combining two anticancer treatments with enhanced therapeutic efficiency." in: The Journal of clinical endocrinology and metabolism, Vol. 95, Issue 2, pp. 943-52, 2010 (PubMed).

Background publications Beauvais, Rapraeger: "Syndecan-1 couples the insulin-like growth factor-1 receptor to inside-out integrin activation." in: Journal of cell science, Vol. 123, Issue Pt 21, pp. 3796-807, 2010 (PubMed).

Li, Kato, Paz, Kasuya, Fujita-Yamaguchi: "Two new monoclonal antibodies against the alpha subunit of the human insulin-like growth factor-I receptor." in: Biochemical and biophysical research communications, Vol. 196, Issue 1, pp. 92-8, 1993 (PubMed).

Did you look for something else?