CD32/CD16 antibody (APC)

Catalog No. ABIN1112436

Product Details anti-CD32/CD16 Antibody (APC)

Target: CD32/CD16

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD32/CD16 antibody is conjugated to APC

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)

Characteristics: Monoclonal purified Anti-Mouse CD16/CD32 is recommended for use in flow cytometry for identification of the Mouse CD16 (Fcgamma III Receptor) and CD32 (Fcgamma II Receptor).

Immunogen: Proprietary peptide secuence

Clone: LK2H10

Isotype: IgG2a

Application Details

Application Notes: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis. Recommeded Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometry analysis. For immunofluorescent staining, the suggested use of this reagent is less or equal to 1 µg per 10^6 cells in 100 µL volume or 100 µL of whole bloodIt is recommended that the reagent be titrated for optimal performance for each application.

Comment:

Allophycocyanin (Abs/Em: 651/ 662 nm).

Reagent Preparation:

1. Transfer the sample to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add antibody and mix gently with a vortex mixer. The optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive purified antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 mL of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 mL 0.01 mol/L PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 mL PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The conjugate is provided Phosphate-buffered solution, pH 7.2, containing 0.09% Sodium azide.

Preservative: Sodium azide

Precaution of Use: 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Storage: 4 °C

Target Details for CD32/CD16

Target: CD32/CD16

Alternative Name: CD16/CD32 (CD32/CD16 Products)

Background: The anti-Mouse CD16/CD32 is used in flow cytometric analysis. CD16 is the low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/ macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody- antigen immune complexes and mediate adaptive immune responses. The 93 mAb is specific to the common epitope of CD16/CD32. It is useful for blocking non-specific binding of immunoglobulin to the Fc receptors.This clone 93, has been reported in blocking of Fc- mediated reaction in functional studies.