CD45.2 antibody (APC)

Catalog No. ABIN1112456

Product Details anti-CD45.2 Antibody (APC)

Target: CD45.2 (CD45 (CD45.2 Allogen))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD45.2 antibody is conjugated to APC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Characteristics: Mouse monoclonal Anti-Mouse CD45.2 APC is recommended for use in flow cytometry for identification of mouse CD45.2, an alloantigen of CD45 expressed by Ly5.2 bearing mouse strains.

Immunogen: B10.S mouse thymocytes and splenocytes

Clone: Ks20-8

Isotype: IgG2a

Application Details

Application Notes: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of mouse tissue for flow cytometric analysis using less than or equal to less than or equal to 0,25 µg/10^6 cells in 100 µL volumeIt is recommended that the reagent be titrated for optimal performance for each application.

Comment:

Allophycocyanin (APC). Abs/Em: 651/ 662 nm.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The conjugate is provided in liquid form in buffer containing PBS and 0,09% Sodium azide, pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for CD45.2

Target: CD45.2 (CD45 (CD45.2 Allogen))

Alternative Name: CD45.2 (CD45.2 Products)

Background: CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kDa glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell, as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.