Insulin antibody

Catalog No. ABIN111464

The Mouse Monoclonal anti-Insulin antibody has been validated for IHC (p), IHC (fro) and EIA. It is suitable to detect Insulin in samples from Human. There is 1 publication available.

Quick Overview for Insulin antibody (ABIN111464)

Target: Insulin (INS)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Insulin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: C-PEP-01

Product Details anti-Insulin Antibody

Specificity: This antibody recognises C-peptide (also known as connecting peptide) a peptide released when proinsulin is cleaved to produce insulin. It can also be used to detect proinsulin.

Cross-Reactivity (Details): Also reacts to: Proinsulin

Purification: Affinity chromatography on Protein A

Immunogen: Human C-peptide conjugated to BSA

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Insulin antibody (ABIN111464)

Meier, Köhler, Alkhatib, Sergi, Junker, Klein, Schmidt, Fritsch: "Beta-cell development and turnover during prenatal life in humans." in: European journal of endocrinology, Vol. 162, Issue 3, pp. 559-68, (2010) (PubMed).

Target Details for Insulin

Target: Insulin (INS)

Alternative Name: Insulin

Background: Synonyms: INS

Gene ID: 3630

NCBI Accession: NP_000198

UniProt: P01308

Pathways: NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation