STAT5A antibody (pTyr694)

Catalog No. ABIN1118522

Supplier Product No.: 200-301-b18

The Mouse Monoclonal anti-STAT5A antibody has been validated for WB, IHC, ELISA, IF and FM. It is suitable to detect STAT5A in samples from Human and Mouse.

Quick Overview for STAT5A antibody (pTyr694) (ABIN1118522)

Target: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This STAT5A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Clone: 5F6-F1

Product Details anti-STAT5A Antibody

Binding Specificity: pTyr694

Supplier Product No.: 200-301-b18

Supplier: Rockland

Purpose: Stat5 phospho Y694 Antibody

Cross-Reactivity (Details): This antibody is specific for mouse Stat5a protein phosphorylated at Y694.

Characteristics: Synonyms: mouse anti-STAT5 pY694 Antibody, MGF antibody, Signal Transducer and Activator of Transcription 5A antibody, STAT 5 antibody

Purification: Anti-STAT5 phospho Y694 Antibody was purified from concentrated tissue culture supernate by Protein A chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Phospho STAT5 pY694 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5a protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG1 kappa

Application Details

Application Notes:

Immunohistochemistry Dilution: 20-40 μg/mL

Application Note: This monoclonal antibody has been tested by ELISA, immunofluorescence, immunohistochemistry, and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 91 kDa in size corresponding to phosphorylated Stat5a protein by western blotting in the appropriate cell lysate or extract.  This phospho-specific monoclonal antibody reacts with mouse Stat5a phospho Y694 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:20,000

IF Microscopy Dilution: 1:50 - 1:1,000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store Anti-STAT5 phospho Y694 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Phospho STAT5 pY694 Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for STAT5A

Target: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Alternative Name: Stat5a

Background: Background: Signal transducer and activator of transcription 5 (Stat5) belongs to a family of cytoplasmic transcription factors that can be activated (phosphorylated) by a cell surface receptor. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Stat5 has two isoforms, Stat5 alpha and Stat5 beta. Aberrant Stat5 activation has been implicated in the pathogenesis of chronic myelogenous leukemia, prostate and breast cancer and tumor metastasis. Stat5 is localized in the cytoplasm and upon phosphorylation at Y694 is translocated to the nucleus. Ideal for Cancer, Chromatin & Nuclear Signaling and Signal Transduction research.

Gene ID: 20850, 6755672

UniProt: P42230

Pathways: JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation