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Vimentin antibody

VIM Reactivity: Human, Mouse, Rat, Pig, Chicken, Monkey, Dog, Goat, Hamster, Zebrafish (Danio rerio) WB, IF, FACS, IHC (p), IHC (fro) Host: Mouse Monoclonal RV202 unconjugated
Catalog No. ABIN112354
  • Target See all Vimentin (VIM) Antibodies
    Vimentin (VIM)
    Reactivity
    • 332
    • 125
    • 124
    • 60
    • 58
    • 42
    • 41
    • 35
    • 29
    • 20
    • 20
    • 13
    • 12
    • 8
    • 6
    • 6
    • 6
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Pig, Chicken, Monkey, Dog, Goat, Hamster, Zebrafish (Danio rerio)
    Host
    • 190
    • 128
    • 9
    • 6
    • 4
    • 4
    • 2
    Mouse
    Clonality
    • 198
    • 144
    Monoclonal
    Conjugate
    • 199
    • 19
    • 13
    • 13
    • 11
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    This Vimentin antibody is un-conjugated
    Application
    • 281
    • 131
    • 98
    • 98
    • 96
    • 64
    • 49
    • 40
    • 39
    • 27
    • 23
    • 20
    • 8
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificity
    RV202 reacts exclusively with Vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.
    Cross-Reactivity (Details)
    Species reactivity (tested):Canine, Chicken, Goat, Hamster, Human, Monkey, Mouse, Rat, Swine, Zebrafish.
    Purification
    Purified
    Immunogen
    Cytoskeletal vimentin extract of calf lens.
    Clone
    RV202
    Isotype
    IgG1
    Top Product
    Discover our top product VIM Primary Antibody
  • Application Notes
    RV202 is suitable for Immunoblotting, Immunocytochemistry, Immunohistochemistry onfrozen tissues and Flow cytometry. Recommended Dilutions: 1/100-1/200 for Flow cytometry, and for Immunohistochemistrywith avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and1/100-1/1000 for Immunoblotting applications.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    PBS, 0.09 % Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the lyophilized antibody at 2-8 °C for one month or at -20 °C for longer. Aliquot and store the reconstituted antibody at -20 °C.
  • Target
    Vimentin (VIM)
    Alternative Name
    Vimentin (VIM Products)
    Synonyms
    CTRCT30 antibody, cb28 antibody, vime antibody, vim antibody, vim1 antibody, vim2 antibody, VIM antibody, Vimentin antibody, vim4 antibody, vimentin antibody, vimentin L homeolog antibody, vimentin S homeolog antibody, VIM antibody, Vim antibody, vim antibody, vim.L antibody, vim.S antibody
    Background
    Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.Synonyms: VIM
    Gene ID
    7431
    NCBI Accession
    NP_003371
    UniProt
    P08670
    Pathways
    Caspase Cascade in Apoptosis
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