Collagen Type I antibody

Catalog No. ABIN113959

This Rabbit Polyclonal antibody specifically detects Collagen Type I in IHC (fro), IF, IHC (p), RIA and EIA. It exhibits reactivity toward Fish, Tunafish and Bonito.

Quick Overview for Collagen Type I antibody (ABIN113959)

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reactivity: Fish, Tunafish, Bonito

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen Type I antibody is un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA), Enzyme Immunoassay (EIA)

Product Details anti-Collagen Type I Antibody

Specificity: This antibody is specific for Fish Collagen type I (Tuna Fish).

Purification: Affinity Chromatography

Immunogen: Purified Collagen type I from Tuna Fish skin

Application Details

Application Notes: ELISA. RIA. Immunofluorescence: 1/40. Immunohistochemistry on Paraffin Sections: 1/200-1/600 for 60 min at RT or 2-8 °C overnight. Positive control: Tuna Fish skin. Pretreatment: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase(app.300 U/mg) in TBS 15 min at 37 °C. There after non-specific binding is blocked byblocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxidesolution in TBS for 30 min at RT is recommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.1 mL distilled water, for further dilution use appropriate antibody diluent

Concentration: Approx. 1 mg/mL

Buffer: Phosphate Buffered solution, None, None

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Collagen Type I

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Alternative Name: Collagen Type I

Background: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon.Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2

Gene ID: 1277

NCBI Accession: NP_000079

UniProt: P02452