Collagen IV antibody

Catalog No. ABIN113972

The Rabbit Polyclonal anti-Collagen IV antibody has been validated for IHC (fro), IHC (p), IF, EIA and RIA. It is suitable to detect Collagen IV in samples from Pig.

Quick Overview for Collagen IV antibody (ABIN113972)

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen IV antibody is un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA)

Product Details anti-Collagen IV Antibody

Specificity: This antibody is specific for Collagen type IV (100 %). Porcine Collagen type I, II, III, Elastin, Fibronectin and Laminin < 0.1 % (in RIA at 1/1000 dilution).

Purification: Affinity Chromatography.

Immunogen: Purified collagen type IV from porcine renals.

Application Details

Application Notes: ELISA: > 1/200 gives OD > 500. RIA. Immunofluorescence (1/80). Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 1/500. Incubation time: 60 min. at RT or 2-8 °C overnight. Positive Control: Porcine renals. Pretreatment: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase(app.300 U/mg) in TBS 15 min at 37 °C. There after non-specific binding is blocked byblocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxidesolution in TBS for 30 min at RT is recommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water. For further dilution use appropriate antibody diluent.

Concentration: 1.0 mg/mL

Buffer: PBS without BSA and preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Alternative Name: Collagen Type IV

Background: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type IV Collagen (170-180 kDa), is a non-fibrilary network of different alpha-chains. It is typically found in basal membranes of different organs (e.g. skin, lens, lung, renals).Synonyms: COL4A1

Gene ID: 1282

NCBI Accession: NP_001836

UniProt: P02462