Androgen Receptor antibody (AA 340-356)

Catalog No. ABIN114765

This anti-Androgen Receptor antibody is a Mouse Monoclonal antibody detecting Androgen Receptor in WB, IHC (p) and IHC (fro). Suitable for Human and Dog.

Quick Overview for Androgen Receptor antibody (AA 340-356) (ABIN114765)

Target: Androgen Receptor (AR)

Reactivity: Human, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Androgen Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: AR441

Product Details anti-Androgen Receptor Antibody

Binding Specificity: AA 340-356

Specificity: This antibody is specific to a protein of 110 kDa, identified as androgen receptor. This antibody reacts with full length of AR and also with the newly described A form of the receptor. This antibody does not cross react with estrogen, progesterone or glucocorticoid receptors.
Cellular Localization: Nuclear. Has been reported to cross-react with Dog.

Purification: Purified

Immunogen: BALB/C mice were injected with a synthetic peptide, amino acid 340-356 (STEDTAEYSPFKGGYTK) of Human Androgen Receptor

Isotype: IgG1

Application Details

Application Notes: Western Blot: 1: 50 to 1: 100. Immunohistochemistry on paraffin embedded sections: dilute 1: 25-1: 50 in an ABC method(30 minutes at room temperature). Formalin fixed paraffin embedded tissue sectionsrequire high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior toimmunostaining. Recommended Positive Control: Prostate carcinoma
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Androgen Receptor

Target: Androgen Receptor (AR)

Alternative Name: Androgen Receptor

Background: The androgen receptor (AR) is a 110 kDa androgen-dependent transcription factor that is a member of the steroid/nuclear receptor gene superfamily. The AR signaling pathway plays a key role in development and function of male reproductive organs, including the prostate and epididymis. AR also plays a role in nonreproductive organs, such as muscle, hair follicles, and brain. Abnormalities in the AR signaling pathway have been linked to a number of diseases, including prostate cancer, Kennedy's disease and male infertility. The PI3K/Akt signaling pathway plays an important role in regulating AR activity through phosphorylation of AR at Ser213/210 and Ser791/790. Growth factors or cytokines may induce phosphorylation of AR through the PI3K/Akt pathway. Activation of the PI3K/AKt pathway is thought to have a survival role in prostate cancer by protecting cells from apoptosis.Synonyms: DHTR, Dihydrotestosterone receptor, NR3C4, Nuclear receptor subfamily 3 group C member 4

Gene ID: 367

NCBI Accession: NP_000035

UniProt: P10275

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding