TEK antibody (Extracellular Domain)

Catalog No. ABIN115159

This Mouse Monoclonal antibody specifically detects TEK in WB, FACS, IHC (fro) and EIA. It exhibits reactivity toward Human.

Quick Overview for TEK antibody (Extracellular Domain) (ABIN115159)

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TEK antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: Cl-16

Product Details anti-TEK Antibody

Binding Specificity: Extracellular Domain

Specificity: The monoclonal antibody will detect native Human TIE-2/tek in ELISA experiments and on the surface of different cell types.

Purification: Protein G Affinity Chromatoghraphy

Immunogen: Recombinant Human soluble extracellular TIE-2 protein

Isotype: IgG1

Application Details

Application Notes: ELISA: 1-15 μg/mL. Western blotting: 1-2 μg/mL. Flow cytometry and cell sorting (FACS): 2-5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.4, without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freeze-thaw cycles!

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antibody, thought stable at room temperature, is best stored desiccated below 0 °C. Reconstituted anti-TIE-2/tek is stable at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for TEK

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Alternative Name: CD202b / TEK

Background: TIE2 (tyrosine kinase with Ig and EGF homology domains 2) is expressed almost exclusively in endothelial cells in mice, rats and humans. This receptor possesses a unique extracellular domain containing two immunoglobulin like loops separated by three epidermal growth factor like repeats that are connected to three fibronectin type III like repeats. The ligand for the receptor is Angiopoietin 1. Defects in TIE2 are associated with inherited venous malformations, the TIE2 signaling pathway appears to be critical for endothelial cell smooth muscle cell communication in venous morphogenesis.Synonyms: Angiopoietin-1 receptor, TIE-2, TIE2, Tunica interna endothelial cell kinase, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, p140 TEK

Gene ID: 7010

NCBI Accession: NP_000450

UniProt: Q02763

Pathways: RTK Signaling, Growth Factor Binding